Lundberg U, Nilsson G, von Gabain A
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.
Gene. 1988 Dec 10;72(1-2):141-9. doi: 10.1016/0378-1119(88)90136-9.
Using two monocistronic gene transcripts, bla and ompA, we have studied the relationship between mRNA stability and translational efficiency. It was found that changes in the ompA mRNA stability are not correlated with an alteration in translational efficiency. In addition, at slow bacterial growth rates, the ompA transcript is translated ten times more efficiently than the bla messenger although the stability of the two transcripts is about equal. At rapid bacterial growth rate, chloramphenicol slightly stabilises both the bla and ompA transcripts without affecting their characteristic difference in half-life. Thus, control of mRNA stability seems not necessarily to be mediated either by the efficiency of loading ribosomes on a transcript, or by the arrest or slowing down of translating ribosomes.
利用两个单顺反子基因转录本bla和ompA，我们研究了mRNA稳定性与翻译效率之间的关系。结果发现，ompA mRNA稳定性的变化与翻译效率的改变无关。此外，在细菌生长缓慢时，尽管两种转录本的稳定性大致相同，但ompA转录本的翻译效率比bla信使RNA高10倍。在细菌快速生长时，氯霉素略微稳定了bla和ompA转录本，但不影响它们在半衰期上的特征差异。因此，mRNA稳定性的控制似乎不一定是由核糖体在转录本上的加载效率介导的，也不是由翻译核糖体的停滞或减慢介导的。
