College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Qing Hua Dong Lu, Beijing, 100083, China; Guangdong Academy of Forestry, Guangzhou, 510520, China.
College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Qing Hua Dong Lu, Beijing, 100083, China.
J Plant Physiol. 2022 Apr;271:153644. doi: 10.1016/j.jplph.2022.153644. Epub 2022 Feb 10.
Heterophylly is regard as adaptation to different environments in plant, and Populus euphratica is an important heterophyllous woody plant. However, information on its molecular mechanism in eco-adaptability remains obscure.
In this research, proteins were identified by isobaric tags for relative and absolute quantitation (iTRAQ) technology in lanceolate, ovate, and dentate broad-ovate leaves from adult P. euphratica trees, respectively. Besides, chlorophyll content, net photosynthetic rate, stomatal conductance, transpiration rate and peroxidase activity in these heteromorphic leaves were investigated. A total number of 2,689 proteins were detected in the heteromorphic leaves, of which 56, 73, and 222 differential abundance proteins (DAPs) were determined in ovate/lanceolate, dentate broad-ovate/lanceolate, and dentate broad-ovate/ovate comparison groups. Bioinformatics analysis suggested these altered proteins related to photosynthesis, stress tolerance, respiration and primary metabolism accumulated in dentate broad-ovate and ovate leaves, which were consistent with the results of physiological parameters and Real-time Quantitative PCR experiments.
This research demonstrated the mechanism of the differential abundance proteins in providing an optimal strategy of resource utilization and survival for P. euphratica, that could offer clues for further investigations into eco-adaptability of heterophyllous woody plants.
异形叶性被认为是植物适应不同环境的一种特性，胡杨是一种重要的异形叶木本植物。然而，其在生态适应性方面的分子机制信息仍然不清楚。
本研究分别采用等重同位素标记相对和绝对定量（iTRAQ）技术，鉴定了成年胡杨披针形、卵形和齿状宽卵形叶片中的蛋白质。此外，还研究了这些异形叶中的叶绿素含量、净光合速率、气孔导度、蒸腾速率和过氧化物酶活性。在异形叶中共检测到 2689 种蛋白质，其中在卵形/披针形、齿状宽卵形/披针形和齿状宽卵形/卵形比较组中分别确定了 56、73 和 222 种差异丰度蛋白（DAP）。生物信息学分析表明，这些改变的蛋白质与光合作用、胁迫耐受、呼吸和初级代谢有关，在齿状宽卵形和卵形叶片中积累，这与生理参数和实时定量 PCR 实验的结果一致。
本研究证明了差异丰度蛋白在为胡杨提供最佳资源利用和生存策略方面的机制，为进一步研究异形叶木本植物的生态适应性提供了线索。
