College of Life Sciences, Tarim University, Alar 843300, China.
College of Life Sciences, Tarim University, Alar 843300, China; Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar 843300, China.
Genomics. 2020 Jan;112(1):92-98. doi: 10.1016/j.ygeno.2019.01.013. Epub 2019 Jan 29.
Populus euphratica Oliv. has typical heterophylly. Linear, lanceolate, ovate and broad-ovate leaves appeared in turn from sprouting to development, to maturity. The environmental adaptabilities of P. euphraticas with different leaves were also different. To explore the role of circRNAs on the morphogenesis of P. euphratica heteromorphic leaves (P.hl) and their stress response, the expression profile of circRNAs was analyzed by strand-specific RNA sequencing for the above four kinds of heteromorphic leaves. According to ceRNA hypothesis, 18 differentially expressed cirRNAs (DECs) could influence the expression of 84 mRNAs by antagonizing 23 miRNAs in five sample-pairs. Based on the function of 84 mRNAs, these DECs participate in development process, response to stimulus, response to hormonal et al. Therefore, these circRNAs were involved in the P.hl morphogenesis and stress response by interacting with miRNAs and mRNAs. Our study complemented the genebank of P. euphratica and provided a new strategy for studying leaf development.
胡杨具有典型的异形叶性。从萌芽到发育再到成熟，依次出现了线性、披针形、卵形和宽卵形的叶子。不同叶型胡杨的环境适应能力也不同。为了探讨 circRNAs 在胡杨异形叶（P.hl）形态发生及其胁迫响应中的作用，本研究通过链特异性 RNA 测序对上述四种异形叶进行了 circRNAs 表达谱分析。根据 ceRNA 假说，在五个样本对中，18 个差异表达的 cirRNAs（DECs）可以通过拮抗 23 个 miRNA 来影响 84 个 mRNAs 的表达。基于 84 个 mRNAs 的功能，这些 DECs 通过与 miRNA 和 mRNAs 的相互作用参与了发育过程、对刺激的反应、对激素的反应等。因此，这些 circRNAs 通过与 miRNA 和 mRNAs 的相互作用参与了 P.hl 的形态发生和胁迫响应。本研究补充了胡杨的基因库，并为研究叶片发育提供了新的策略。
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