Jiangsu Key Laboratory of Pesticide Science, College of Sciences, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, China.
Jiangsu Key Laboratory of Pesticide Science, College of Sciences, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, China.
Ecotoxicol Environ Saf. 2022 Mar 15;233:113349. doi: 10.1016/j.ecoenv.2022.113349. Epub 2022 Feb 24.
The plant acetyltransferases (ACEs) belong to a super family of proteins that contribute to secondary metabolisms and involve various abiotic and biotic stress responses. However, how rice ACEs respond to toxic agrochemicals is largely unknown. This study demonstrates that 86 and 83 genes coding ACEs in the transcriptome profiling were expressed under mesotrione (MTR) and fomesafen (FSA) exposure, respectively. Of these, 18 and 8 ACE differentially expressed genes (DEGs) were identified in MTR- and FSA-exposed rice transcriptome datasets. Some of the ACE genes were validated by quantitative RT-PCR analysis. Analysis of biochemical properties of ACEs revealed that many genes have various cis-elements and structural domain which may cope with a variety of biotic and abiotic stress responses and detoxification of xenobiotics. Moreover, the ACE activities in rice were induced under MTR and FSA exposure and reached out to the highest value at the 0.1 mg L. The ACE activities in the MTR and FSA treated roots were 2.6 and 3.5 fold over the control and those in shoots with MTR and FSA were 4.0 and 26.1 fold over the control, respectively. These results indicate that the ACE-coding genes can respond to the MTR and FSA stress by increasing their transcriptional level, along with the enhanced specific ACE protein activities in rice tissues.
植物乙酰基转移酶（ACEs）属于蛋白质的超家族，有助于次生代谢，并参与各种非生物和生物胁迫反应。然而，水稻 ACEs 如何响应有毒农用化学品在很大程度上是未知的。本研究表明，在草丁膦（MTR）和氟磺胺草醚（FSA）暴露下，分别有 86 和 83 个基因编码的 ACE 在转录组分析中表达。其中，在 MTR 和 FSA 暴露的水稻转录组数据集中分别鉴定出 18 和 8 个 ACE 差异表达基因（DEGs）。一些 ACE 基因通过定量 RT-PCR 分析进行了验证。对 ACE 基因的生化特性分析表明，许多基因具有各种顺式元件和结构域，可能与各种生物和非生物胁迫反应以及对外源化合物的解毒有关。此外，在 MTR 和 FSA 暴露下，水稻中的 ACE 活性被诱导，在 0.1mg/L 时达到最高值。MTR 和 FSA 处理的根部中的 ACE 活性分别比对照增加了 2.6 倍和 3.5 倍，而在茎部中，MTR 和 FSA 的 ACE 活性分别比对照增加了 4.0 倍和 26.1 倍。这些结果表明，ACE 编码基因可以通过增加其转录水平来响应 MTR 和 FSA 胁迫，同时提高水稻组织中特定的 ACE 蛋白活性。
