纤溶酶原受体通过靶向 BMI1 指导精原干细胞的维持。
The plasminogen receptor directs maintenance of spermatogonial stem cells by targeting BMI1.
机构信息
Human Reproductive and Genetic Center, Affiliated Hospital of Jiangnan University, Wuxi, 214122, China.
State Key Laboratory of Reproductive Medicine, Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, 215002, China.
出版信息
Mol Biol Rep. 2022 Jun;49(6):4469-4478. doi: 10.1007/s11033-022-07289-1. Epub 2022 Feb 27.
BACKGROUND
Spermatogonial stem cells (SSCs) are unique stem cells that account for the whole reproductive life of males and transmit genetic information to offspring. SSC maintenance is intricate and the underlying mechanisms are largely unclear. Here, we report that SSC maintenance is driven by the plasminogen receptor (PLGRKT).
METHODS AND RESULTS
PLGRKT was located in SSCs, and knockdown of PLGRKT expression in cultured neonatal testis and SSCs impaired the proliferation and promoted the apoptosis of cells. PLGRKT interacted with B lymphoma Mo-MLV insertion region 1 (BMI1), and modulated oxidative stress and p16/p19 signaling in SSCs.
CONCLUSIONS
We demonstrated that reactive oxygen species (ROS) and p16/p19 signaling are involved in "PLGRKT-BMI1" co-regulation of SSC maintenance in mice.
背景
精原干细胞（SSCs）是独特的干细胞，它们构成了男性的整个生殖生命，并将遗传信息传递给后代。SSC 的维持非常复杂，其潜在机制在很大程度上尚不清楚。在这里，我们报告说，PLGRKT 驱动 SSC 的维持。
方法和结果
PLGRKT 位于 SSCs 中，在培养的新生睾丸和 SSCs 中敲低 PLGRKT 的表达会损害细胞的增殖并促进细胞凋亡。PLGRKT 与 B 淋巴瘤 Mo-MLV 插入区 1（BMI1）相互作用，并调节 SSCs 中的氧化应激和 p16/p19 信号。
结论
我们证明 ROS 和 p16/p19 信号参与了“PLGRKT-BMI1”对小鼠 SSC 维持的共同调节。
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