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周期 2 通过上皮-间充质转化过程抑制结直肠癌的恶性细胞行为。

Period 2 Suppresses the Malignant Cellular Behaviors of Colorectal Cancer Through the Epithelial-Mesenchymal Transformation Process.

机构信息

Department of Gastrointestinal Surgery, 12466Affiliated Zhongshan Hospital of Xiamen UniversityUniversity, Xiamen, China.

School of Medicine, 12466Xiamen UniversityUniversity, Xiamen, China.

出版信息

Cancer Control. 2022 Jan-Dec;29:10732748221081369. doi: 10.1177/10732748221081369.

DOI:10.1177/10732748221081369
PMID:35220799
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8891940/
Abstract

INTRODUCTION

The PER2 (Period circadian regulator 2) gene is related to the circadian clock, and it has been deemed as a suppressor gene in osteosarcoma and lung carcinoma. However, the part of PER2 in CRC (colorectal cancer) needs to be further determined.

METHODS

First, we collected clinical samples to detect PER2 expression in CRC. Then, we used cell transfection to knock down PER2 expression in CRC cell lines and performed a series of functional experiments to elucidate the effects of PER2 on CRC cells. We next verified whether PER2 affects the epithelial-mesenchymal transformation (EMT) process in CRC by conducting quantitative real-time PCR and western blotting.

RESULTS

In the research, we revealed that the expression of PER2 decreased in CRC clinical samples. In addition, knocking down PER2 expression caused CRC cells to acquire malignant biological features. Finally, we found that PER2 knockdown may activate the Snail/Slug axis through inhibiting p53, therefore promote the activation of the EMT pathway.

CONCLUSION

In conclusion, low PER2 expression reinforces migration and activates EMT in CRC, suggesting that PER2 is closely related to CRC development and could be used as a potential treatment site in the clinic.

摘要

简介

PER2（Period circadian regulator 2）基因与生物钟有关，它被认为是骨肉瘤和肺癌的抑制基因。然而，PER2 在 CRC（结直肠癌）中的作用仍需进一步确定。

方法

首先，我们收集临床样本检测 CRC 中 PER2 的表达。然后，我们通过细胞转染敲低 CRC 细胞系中的 PER2 表达，并进行一系列功能实验阐明 PER2 对 CRC 细胞的影响。接下来，我们通过实时定量 PCR 和 Western blot 验证 PER2 是否影响 CRC 中的上皮-间充质转化（EMT）过程。

结果

在研究中，我们揭示了 CRC 临床样本中 PER2 的表达降低。此外，敲低 PER2 表达使 CRC 细胞获得恶性生物学特征。最后，我们发现 PER2 敲低可能通过抑制 p53 激活 Snail/Slug 轴，从而促进 EMT 通路的激活。

结论

总之，低表达的 PER2 增强了 CRC 细胞的迁移并激活了 EMT，表明 PER2 与 CRC 的发生发展密切相关，可作为临床治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/41558185a595/10.1177_10732748221081369-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/21f0c6965776/10.1177_10732748221081369-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/20070d127a78/10.1177_10732748221081369-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/83699d3ffa5e/10.1177_10732748221081369-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/5970602d2d04/10.1177_10732748221081369-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/4db3e393fbed/10.1177_10732748221081369-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/41558185a595/10.1177_10732748221081369-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/21f0c6965776/10.1177_10732748221081369-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/20070d127a78/10.1177_10732748221081369-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/83699d3ffa5e/10.1177_10732748221081369-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/5970602d2d04/10.1177_10732748221081369-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/4db3e393fbed/10.1177_10732748221081369-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8827/8891940/41558185a595/10.1177_10732748221081369-fig6.jpg

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