在伊朗德黑兰进行的一项研究：粪便样本中的惠尔通氏体在儿童急性腹泻中的作用。
Tropheryma whipplei in the stool samples of children with acute diarrhea: a study from Tehran, Iran.
机构信息
Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran.
National Reference Laboratory for Plague, Tularemia and Q Fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar Ahang, Hamadan, Iran.
出版信息
BMC Infect Dis. 2022 Feb 27;22(1):193. doi: 10.1186/s12879-022-07198-5.
BACKGROUND
Recently, Tropheryma whipplei has been suggested as one of the causative agents of diarrhea among children worldwide. Limited data is available on the prevalence of T. whipplei among children with diarrhea in most countries such as Iran. This study was conducted to evaluate the prevalence of T. whipplei in children with acute diarrhea in Iran.
METHODS
In this study, the stool samples were collected from 130 children under 10 years old with acute diarrhea from children's hospitals in Tehran city. Genomic DNA was extracted from stool samples and was tested for the presence of DNA of T. whipplei using the SYBR Green Real-time PCR method. Positive T. whipplei samples were finally confirmed by PCR Product sequencing.
RESULTS
The mean age of participants was 32.5 months, and 54.6% of children were female. Using the SYBR Green Real-time PCR, 9.23% (12/130) of samples were positive for T. whipplei, which were confirmed by sequencing. 66.67% of positive cases were males. The duration of diarrhea in infected children with T. whipplei (83.3%) was significantly longer (OR: 5.93, 95% CI 1.24-28.22) compared to children with negative results (45.8%). Other demographic factors and clinical signs had not a statistically significant relationship with T. whipplei infection.
CONCLUSIONS
In this study, T. whipplei was detected in stool samples of children with acute diarrhea. The results indicated that T. whipplei could be associated with childhood diarrhea in Iran. The health care system and physicians should be aware of the presence of T. whipplei infection in Iran, especially in childhood diarrhea.
背景
最近，旋毛虫被认为是全球儿童腹泻的病因之一。在大多数国家，如伊朗，关于腹泻儿童中旋毛虫患病率的数据有限。本研究旨在评估伊朗急性腹泻儿童中旋毛虫的患病率。
方法
本研究从德黑兰市儿童医院的 130 名 10 岁以下急性腹泻儿童中采集粪便样本。从粪便样本中提取基因组 DNA，并使用 SYBR Green 实时 PCR 方法检测 T. whipplei DNA 的存在。最后通过 PCR 产物测序对阳性 T. whipplei 样本进行确认。
结果
参与者的平均年龄为 32.5 个月，其中 54.6%为女性。使用 SYBR Green 实时 PCR，130 份样本中有 9.23%（12/130）为 T. whipplei 阳性，通过测序得到确认。阳性病例中 66.67%为男性。感染旋毛虫的儿童腹泻持续时间（83.3%）明显更长（OR：5.93，95%CI 1.24-28.22），与阴性结果（45.8%）相比。其他人口统计学因素和临床症状与旋毛虫感染无统计学显著关系。
结论
本研究在急性腹泻儿童的粪便样本中检测到了 T. whipplei。结果表明，T. whipplei 可能与伊朗儿童腹泻有关。伊朗的医疗保健系统和医生应意识到 T. whipplei 感染的存在，尤其是在儿童腹泻中。
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