老挝幼儿园儿童中惠普尔嗜组织细胞菌的高流行率。
High prevalence of Tropheryma whipplei in Lao kindergarten children.
作者信息
Keita Alpha Kabinet, Dubot-Pérès Audrey, Phommasone Koukeo, Sibounheuang Bountoy, Vongsouvath Manivanh, Mayxay Mayfong, Raoult Didier, Newton Paul N, Fenollar Florence
机构信息
Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, Marseille, France.
UMR_D 190, Aix Marseille Univ-IRD-EHESP, Medical University, Marseille, France; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR; Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom.
出版信息
PLoS Negl Trop Dis. 2015 Feb 20;9(2):e0003538. doi: 10.1371/journal.pntd.0003538. eCollection 2015 Feb.
BACKGROUND
Tropheryma whipplei is a bacterium commonly found in feces of young children in Africa, but with no data from Asia. We estimated the prevalence of T. whipplei carriage in feces of children in Lao PDR (Laos).
METHODS/PRINCIPAL FINDINGS: Using specific quantitative real-time PCR, followed by genotyping for each positive specimen, we estimated the prevalence of T. whipplei in 113 feces from 106 children in Vientiane, the Lao PDR (Laos). T. whipplei was detected in 48% (51/106) of children. Those aged ≤ 4 years were significantly less frequently positive (17/52, 33%) than older children (34/54, 63%; p< 0.001). Positive samples were genotyped. Eight genotypes were detected including 7 specific to Laos. Genotype 2, previously detected in Europe, was circulating (21% of positive children) in 2 kindergartens (Chompet and Akad). Genotypes 136 and 138 were specific to Chompet (21% and 15.8%, respectively) whereas genotype 139 was specific to Akad (10.55%).
CONCLUSIONS/SIGNIFICANCE: T. whipplei is a widely distributed bacterium, highly prevalent in feces of healthy children in Laos. Further research is needed to identify the public health significance of this finding.
背景
鞭毛菌是一种常见于非洲幼儿粪便中的细菌，但尚无来自亚洲的数据。我们估计了老挝儿童粪便中鞭毛菌的携带率。
方法/主要发现：使用特异性定量实时PCR，随后对每个阳性样本进行基因分型，我们估计了老挝万象106名儿童的113份粪便中鞭毛菌的携带率。在48%（51/106）的儿童中检测到了鞭毛菌。年龄≤4岁的儿童阳性率（17/52，33%）显著低于年龄较大的儿童（34/54，63%；p<0.001）。对阳性样本进行了基因分型。检测到8种基因型，其中7种是老挝特有的。先前在欧洲检测到的基因型2在两所幼儿园（Chompet和Akad）中传播（占阳性儿童的21%）。基因型136和138是Chompet特有的（分别为21%和15.8%），而基因型139是Akad特有的（10.55%）。
结论/意义：鞭毛菌是一种广泛分布的细菌，在老挝健康儿童的粪便中高度流行。需要进一步研究来确定这一发现对公共卫生的意义。
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