Zhang Xiaoling, Bao Jinyu, Lu Xu, Tian Peng, Yang Jie, Wei Yunchun, Li Sheng, Ma Shaoying
College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070 China.
College of Horticulture, Gansu Agricultural University, Lanzhou, 730070 China.
Physiol Mol Biol Plants. 2022 Jan;28(1):51-64. doi: 10.1007/s12298-022-01143-1. Epub 2022 Feb 7.
Sulforaphane (SF) is one of the most effective natural products in preventing and fighting cancer, found in cruciferous plants. In this study, broccoli hairy roots grown for 20 d were used as the experimental material, and it was treated with 500 μmol/L melatonin (MT) for 0, 12 and 32 h to explore the effect of MT on the conversion of glucoraphanin (GRA) to SF. Results showed that the yields of GRA and SF were the largest under MT treatment for 12 h, which were 1.53 and 1.93-fold, respectively, compared to 0 h. However, Myrosinases activity was the highest under MT treatment for 32 h, which was 1.42-fold compared to that of the 0 h. The differential expression of key genes involved in GRA conversion to SF in broccoli hairy roots was identified transcriptome sequencing, and the path of the transformation from GRA to SF was simulated, which provided a theoretical basis for establishing an efficient transformation system from GRA to SF.
萝卜硫素（SF）是十字花科植物中发现的预防和对抗癌症最有效的天然产物之一。在本研究中，以生长20天的西兰花毛状根为实验材料，用500μmol/L褪黑素（MT）处理0、12和32小时，以探究MT对萝卜硫苷（GRA）向SF转化的影响。结果表明，MT处理12小时时GRA和SF产量最高，与0小时相比，分别为其1.53倍和1.93倍。然而，MT处理32小时时黑芥子酶活性最高，与0小时相比为其1.42倍。通过转录组测序鉴定了西兰花毛状根中GRA向SF转化的关键基因的差异表达，并模拟了从GRA到SF的转化途径，为建立从GRA到SF的高效转化体系提供了理论依据。
