Botanical Institute, Biocenter, Cologne University, 50674, Cologne, Germany.
Laboratory for Systems and Synthetic Biology, Wageningen University, 6703 HB, Wageningen, The Netherlands.
Nat Commun. 2017 Dec 14;8(1):2132. doi: 10.1038/s41467-017-02285-7.
Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.
尽管植物发育具有高度的可重复性，但仍存在一些随机性。这种发育随机性可能是由基因表达的噪声引起的。在这里，我们分析了拟南芥中蛋白质表达的波动。使用光转化的 KikGR 标记，我们表明单个细胞的蛋白质表达随时间而波动。我们使用双报告系统研究了标记基因表达的外在和内在噪声。我们报告说，外在噪声高于内在噪声，并且与其他几种组织/细胞类型相比，气孔中的外在噪声明显较低。最后，我们表明，在幼叶、下胚轴和根中，细胞在随机蛋白质表达方面是耦合的，但在成熟叶中则不是。我们的数据表明，基因表达的随机性可以在组织/细胞类型之间变化，并且可以以非细胞自主的方式耦合。
