Ma Le, Gong Qiang, Liu Gaoyu, Chen Jieping, Wang Yu, Luo Peng, Shi Chunmeng
Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing, China.
Department of Hematology, Southwest Hospital, First Affiliated Hospital of the Army Medical University, Chongqing, China.
Dose Response. 2022 Feb 23;20(1):15593258221081317. doi: 10.1177/15593258221081317. eCollection 2022 Jan-Mar.
During a major radiation event, a large number of people need to be rapidly assessed for radiation damage to ensure effective medical treatment and efficient use of medical resources. However, current techniques cannot meet the requirement of rapid detection of large quantities of samples in an emergency. It is essential to develop rapid and accurate radiation biodosimeters in peripheral blood. Here, we identified radiation sensitive genes in mice by RNA sequencing and evaluated their utility as radiation biodosimeters in human cell lines. Mice were subjected to gamma-irradiation with different doses (0-8 Gy, .85 Gy/min), and the tail venous blood was analyzed by RNA sequencing. We have identified 5 genes with significantly differential expression after radiation exposure. We found that positive cofactor 4(PC4) had well correlation with radiation dose in human lymphoblastoid cell line after irradiation. The relative expression of PC4 gene showed a good linear correlation with the radiation dose after 1-5 Gy irradiation (.85 Gy/min). PC4 gene can be rapidly recruited to the DNA damage sites faster than γ-H2AX after radiation in immunofluorescence detection. In conclusion, PC4 may be represented as new radiation biological dosimeter for early assessment.
在重大辐射事件中，需要对大量人员进行快速的辐射损伤评估，以确保有效的医疗救治和医疗资源的高效利用。然而，目前的技术无法满足在紧急情况下快速检测大量样本的需求。开发外周血中快速、准确的辐射生物剂量计至关重要。在此，我们通过RNA测序在小鼠中鉴定出辐射敏感基因，并评估了它们作为人类细胞系中辐射生物剂量计的效用。对小鼠进行不同剂量（0 - 8 Gy，0.85 Gy/min）的γ射线照射，通过RNA测序分析尾静脉血。我们鉴定出5个辐射暴露后表达有显著差异的基因。我们发现，照射后人淋巴母细胞系中阳性辅因子4（PC4）与辐射剂量具有良好的相关性。在1 - 5 Gy照射（0.85 Gy/min）后，PC4基因的相对表达与辐射剂量呈现良好的线性相关。在免疫荧光检测中，辐射后PC4基因可在比γ-H2AX更快的时间内快速募集到DNA损伤位点。总之，PC4可能作为一种新型的辐射生物剂量计用于早期评估。
