Zhu Rong, Zhang Qi, Tang Lan, Zhao Yan, Li Jingbao, Li Fengchao
College of Life Sciences, Hebei University, Baoding, China.
College of Life Sciences, Capital Normal University, Beijing, China.
Front Microbiol. 2022 Feb 10;12:774226. doi: 10.3389/fmicb.2021.774226. eCollection 2021.
Because the original description of , type of the genus, is only based on protargol-impregnated specimens, one of the important living features, namely, the presence/absence of cortical granules, remains unknown so far. In the present work, a detailed investigation of a Chinese population of is carried out using the integrated approaches, and the live morphology, ontogenesis, and molecular information of are presented for the first time. The infraciliature of this population corresponds perfectly with that of the original description. The observation indicates that possesses colorless cortical granules. The most prominent morphogenetic feature of is that the parental adoral zone of membranelles is completely replaced by the newly formed one of the proters. Molecular phylogenetic analysis based on a small subunit ribosomal gene (SSU rDNA) shows that five species are clustered with the species from other six Urostylid genera, namely, , , , , , and . The monophyletic probabilities of the family Bakuellidae, genus , subgenus , and subgenus are rejected by the approximately unbiased test. This study further shows that the family Bakuellidae, genus , and subgenus are all nonmonophyletic groups. In order to establish a reasonable classification system, information on molecular and morphogenesis of more Bakuellids and its related species is urgently needed.
由于该属的原始描述仅基于经原银浸染的标本，其重要的活体特征之一，即皮层颗粒的有无，至今仍不清楚。在本研究中，我们采用综合方法对中国的一个种群进行了详细调查，并首次呈现了该种群的活体形态、个体发育及分子信息。该种群的纤毛下器与原始描述完全相符。活体观察表明该种群具有无色的皮层颗粒。该种群最显著的形态发生特征是亲代的口侧膜带完全被子代新形成的口侧膜带所取代。基于小亚基核糖体基因（SSU rDNA）的分子系统发育分析表明，五个该属物种与来自其他六个尾柱虫属的物种聚类在一起，即[此处六个属名原文缺失]。通过近似无偏检验拒绝了巴库虫科、该属、一个亚属和另一个亚属的单系概率。本研究进一步表明，巴库虫科、该属和一个亚属均为非单系类群。为了建立合理的分类系统，迫切需要更多巴库虫及其相关物种的分子和形态发生信息。
