Dow Allexa, Burger Andrew, Marcantonio Endrei, Prisic Sladjana
School of Life Sciences, University of Hawai'i at Mānoa, Honolulu, HI, United States.
School of Ocean and Earth Science and Technology, University of Hawai'i at Mānoa, Honolulu, HI, United States.
Front Microbiol. 2022 Feb 10;13:811774. doi: 10.3389/fmicb.2022.811774. eCollection 2022.
Zinc ion (Zn) is an essential micronutrient and a potent antioxidant. However, Zn is often limited in the environment. Upon Zn limitation, (basonym: ) undergoes a morphogenesis, which relies on alternative ribosomal proteins (AltRPs); i.e., Zn-independent paralogues of Zn-dependent ribosomal proteins. However, the underlying physiological changes triggered by Zn limitation and how AltRPs contribute to these changes were not known. In this study, we expand the knowledge of mechanisms utilized by to endure Zn limitation, by comparing the transcriptomes and proteomes of Zn-limited and Zn-replete . We further compare, corroborate and contrast our results to those reported for the pathogenic mycobacterium, , which highlighted conservation of the upregulated oxidative stress response when Zn is limited in both mycobacteria. By comparing the multi-omics analysis of a knockout mutant lacking AltRPs (Δ) to the wild type strain, we specify the involvement of AltRPs in the response to Zn limitation. Our results show that AltRP expression in does not affect the conserved oxidative stress response during Zn limitation observed in mycobacteria, but AltRPs do significantly impact expression patterns of numerous genes that may be involved in morphogenesis or other adaptive responses. We conclude that AltRPs are not only important as functional replacements for their Zn-dependent paralogues; they are also involved in the transcriptomic response to the Zn-limited environment.
锌离子（Zn）是一种必需的微量营养素和强效抗氧化剂。然而，锌在环境中往往含量有限。在锌受限的情况下，（基原异名： ）会发生形态发生，这依赖于替代核糖体蛋白（AltRPs）；即锌依赖性核糖体蛋白的锌非依赖性旁系同源物。然而，锌限制引发的潜在生理变化以及AltRPs如何促成这些变化尚不清楚。在本研究中，我们通过比较锌受限和锌充足的 的转录组和蛋白质组，扩展了 用于耐受锌限制的机制的知识。我们进一步将我们的结果与针对致病性分枝杆菌 报道的结果进行比较、证实和对比，后者突出了在两种分枝杆菌中锌受限情况下上调的氧化应激反应的保守性。通过将缺乏AltRPs的敲除突变体（Δ）与 野生型菌株的多组学分析进行比较，我们明确了AltRPs在对锌限制的反应中的作用。我们的结果表明， 中AltRP的表达并不影响分枝杆菌中观察到的锌限制期间保守的氧化应激反应，但AltRPs确实显著影响许多可能参与形态发生或其他适应性反应的基因的表达模式。我们得出结论，AltRPs不仅作为其锌依赖性旁系同源物的功能替代物很重要；它们还参与了对锌受限环境的转录组反应。
