Fu Yating, Liu Ying, Nasiroula Aheli, Wang Qichao, Cao Xinhua
Department of Radiology, Urumqi Stomatological Hospital, Urumqi, Xinjiang Uygur Autonomous Region 830011, P.R. China.
Department of General Special Requirements, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830011, P.R. China.
Exp Ther Med. 2022 Mar;23(3):246. doi: 10.3892/etm.2022.11171. Epub 2022 Jan 28.
Long non-coding RNA (lncRNA) HLA complex group 22 (HCG22) is known to be involved in the occurrence and development of cancer; however, its role in oral squamous cell carcinoma (OSCC) remains unclear. Therefore, the main aim of the present study was to investigate the role and mechanisms of action of lncRNA HCG22 in OSCC cells. The expression levels of lncRNA HCG22 and microRNA (miR)-425-5p in OSCC cells were assessed using reverse transcription-quantitative PCR analysis. Cell proliferation was detected using Cell Counting Kit-8 and colony formation assays. In addition, the expression levels of cell proliferation-related proteins, p27, cyclin E and cyclin-dependent kinase 2, were detected by western blot analysis. The cell invasive ability was detected by Transwell assay, while the cell migratory ability was detected via a wound healing assay. The expression levels of the invasion- and migration-related proteins, MMP2 and MMP9, were measured by western blot analysis. The targeted association between lncRNA HCG22 and miR-425-5p was verified by RNA immunoprecipitation and dual-luciferase reporter assays. The results revealed that lncRNA LCG22 was expressed at low levels, while miR-425-5p was highly expressed in OSCC cell lines, based on bioinformatics analysis. The overexpression of lncRNA HCG22 inhibited the proliferation, invasion and migration of OSCC cells. Moreover, lncRNA HCG22 and miR-425-5p were found to have a direct targeted association, and lncRNA HCG22 inhibited cell proliferation, invasion and migration by targeting miR-425-5p. Collectively, the findings of the present study demonstrated that lncRNA HCG22 may inhibit the proliferation, invasion and migration of OSCC cells by downregulating miR-425-5p expression.
长链非编码RNA（lncRNA）人类白细胞抗原复合体组22（HCG22）已知参与癌症的发生和发展；然而，其在口腔鳞状细胞癌（OSCC）中的作用仍不清楚。因此，本研究的主要目的是探讨lncRNA HCG22在OSCC细胞中的作用及其作用机制。采用逆转录定量PCR分析评估OSCC细胞中lncRNA HCG22和微小RNA（miR）-425-5p的表达水平。使用细胞计数试剂盒-8和集落形成试验检测细胞增殖。此外，通过蛋白质免疫印迹分析检测细胞增殖相关蛋白p27、细胞周期蛋白E和细胞周期蛋白依赖性激酶2的表达水平。通过Transwell试验检测细胞侵袭能力，通过伤口愈合试验检测细胞迁移能力。通过蛋白质免疫印迹分析测量侵袭和迁移相关蛋白MMP2和MMP9的表达水平。通过RNA免疫沉淀和双荧光素酶报告基因试验验证lncRNA HCG22与miR-425-5p之间的靶向关联。结果显示，基于生物信息学分析，lncRNA LCG22在OSCC细胞系中低表达，而miR-425-5p高表达。lncRNA HCG22的过表达抑制了OSCC细胞的增殖、侵袭和迁移。此外，发现lncRNA HCG22与miR-425-5p有直接的靶向关联，并且lncRNA HCG22通过靶向miR-425-5p抑制细胞增殖、侵袭和迁移。本研究结果共同表明，lncRNA HCG22可能通过下调miR-425-5p的表达来抑制OSCC细胞的增殖、侵袭和迁移。
