Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Botany, Bacha Khan University, Charsadda, Khyber Pakhtunkhwa, Pakistan.
Oxid Med Cell Longev. 2022 Feb 16;2022:9366223. doi: 10.1155/2022/9366223. eCollection 2022.
Present study established the biological potential of , and extracts for their potential applications in drug formulations. Initially, FTIR was performed to ascertain functional groups and then plant extracts were prepared using five solvents depending on the polarity. Total phenolic contents were observed in the range of 36.36 ± 1.08 mg GAE/g to 95.55 ± 2.46 mg GAE/g while flavonoid contents were found in the range of 10.51 ± 0.25 mg QE/g to 22.17 ± 1.79 mg QE/g. Antioxidant activity was determined using TRP, CUPRAC, TAC and DPPH assays and was recorded highest in followed by extracts. TPC and TFC were found to be strongly correlated with TRP (r > 0.50), CUPRAC (r > 0.53) and DPPH (r = 0.31 and 0.72) assay while weakly correlated with TAC (r = 0.08 and 0.03) as determined by Pearson correlation analysis. Anticancer activity showed that chloroform extracts possess highest cell viability (85.04 ± 4.24%) against HepG2 cell lines while chloroform extracts exhibited highest activity (82.80 ± 2.68%) against HT144 cell lines. Afterwards, highest PXR activation was observed in (3.49 ± 0.34 g/mL fold) at 60 g/mL and was correlated with increase in CYPA activity (15.0 ± 3.00 g/mL IC value). Furthermore, antimalarial activity revealed >47600 IC value against D6 and W2 and antimicrobial assay indicated highest activity (32 ± 2.80 mm) in against . At the end, GC-MS analysis of n-hexane plant extracts showed 99.104% of total identified compounds in and 94.31% in . In conclusion, present study provides insight about the different biological potentials of and extracts that rationalize the applications of these extracts in functional foods and herbal drugs for the management of oxidative-stress related diseases, antimicrobial infections and liver and skin cancer.
本研究旨在确定 和 提取物的生物潜力，以评估其在药物制剂中的潜在应用。首先，通过傅里叶变换红外光谱（FTIR）确定功能基团，然后根据极性使用五种溶剂制备植物提取物。总酚含量范围为 36.36±1.08 mg GAE/g 至 95.55±2.46 mg GAE/g，而总黄酮含量范围为 10.51±0.25 mg QE/g 至 22.17±1.79 mg QE/g。采用 TRP、CUPRAC、TAC 和 DPPH 测定法测定抗氧化活性，结果表明 提取物的抗氧化活性最高，其次是 提取物。TPC 和 TFC 与 TRP（r>0.50）、CUPRAC（r>0.53）和 DPPH（r=0.31 和 0.72）测定法呈强相关性，与 TAC（r=0.08 和 0.03）呈弱相关性，这是通过皮尔逊相关性分析确定的。抗癌活性表明， 氯仿提取物对 HepG2 细胞系的细胞活力最高（85.04±4.24%），而 氯仿提取物对 HT144 细胞系的活性最高（82.80±2.68%）。随后，在 60μg/mL 时， （3.49±0.34μg/mL 倍）中观察到最高的 PXR 激活，并与 CYP450A 活性增加（15.0±3.00μg/mL IC 值）相关。此外，抗疟活性显示对 D6 和 W2 的 IC 值>47600，抗菌试验表明 对 的活性最高（32±2.80mm）。最后，正己烷植物提取物的 GC-MS 分析显示， 中的总鉴定化合物为 99.104%， 中的为 94.31%。总之，本研究提供了有关 和 提取物不同生物潜力的见解，这为这些提取物在功能性食品和草药药物中的应用提供了依据，可用于治疗与氧化应激相关的疾病、抗菌感染以及肝癌和皮肤癌。
