铁皮石斛提取物的抗氧化和细胞毒性活性及 GC-MS 检测相关化合物。
Antioxidant and cytotoxic activities of Dendrobium moniliforme extracts and the detection of related compounds by GC-MS.
机构信息
Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
Annapurna Research Center, Kathmandu, Nepal.
出版信息
BMC Complement Altern Med. 2018 Apr 23;18(1):134. doi: 10.1186/s12906-018-2197-6.
BACKGROUND
The medicinal orchid Dendrobium moniliforme contains water-soluble polysaccharides, phenanthrenes, bibenzyl derivatives, and polyphenol compounds. This study explored the antioxidant and cytotoxic activities of D. moniliforme extracts and detected their bioactive compounds.
METHODS
Plant material was collected from the Daman of Makawanpur district in central Nepal. Plant extracts were prepared from stems using hexane, chloroform, acetone, ethanol and methanol. The total polyphenol content (TPC) in each extract was determined using Folin-Ciocalteu's reagent and the total flavonoid content (TFC) in each extract was determined using the aluminium chloride method. The in vitro antioxidant and cytotoxic activities of each extract were determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays respectively. Gas chromatography and mass spectrometry (GC-MS) analysis was used to detect bioactive compounds.
RESULTS
TPC content was highest (116.65 μg GAE/mg of extract) in D. moniliforme chloroform extract (DMC) and TFC content was highest (116.67 μg QE/mg of extract) in D. moniliforme acetone extract (DMA). D. moniliforme hexane extract (DMH) extract showed the highest percentage of DPPH radical scavenging activity (94.48%), followed closely by D. moniliforme ethanol extract (DME) (94.45%), DMA (93.71%) and DMC (94.35%) at 800 μg/ml concentration. The antioxidant capacities of DMC, DMA, DMH and DME, which were measured in IC values, were much lower 42.39 μg/ml, 49.56 μg/ml, 52.68 μg/ml, and 58.77 μg/ml respectively than the IC of D. moniliforme methanol extract (DMM) (223.15 μg/ml). DMM at the concentration of 800 μg/ml most inhibited the growth of HeLa cells (78.68%) and DME at the same concentration most inhibited the growth of U251 cells (51.95%). The cytotoxic capacity (IC) of DMM against HeLa cells was 155.80 μg/ml of extract and that of DME against the U251 cells was 772.50 μg/ml of extract. A number of bioactive compounds were detected in both DME and DMM.
CONCLUSION
The fact that plant extract of D. moniliforme has a number of bioactive compounds which showed antioxidant and cytotoxic activities suggests the potential pharmacological importance of this plant.
背景
药用兰花铁皮石斛含有水溶性多糖、菲类、双苄基衍生物和多酚化合物。本研究旨在探讨铁皮石斛提取物的抗氧化和细胞毒性活性，并检测其生物活性化合物。
方法
从尼泊尔中部马坎普尔区的大曼采集植物材料。采用正己烷、氯仿、丙酮、乙醇和甲醇从茎中提取植物提取物。使用 Folin-Ciocalteu 试剂测定各提取物中的总多酚含量（TPC），并使用三氯化铝法测定各提取物中的总黄酮含量（TFC）。分别使用 DPPH（2,2-二苯基-1-苦基肼）和 MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物）测定各提取物的体外抗氧化和细胞毒性活性。使用气相色谱和质谱（GC-MS）分析检测生物活性化合物。
结果
铁皮石斛氯仿提取物（DMC）的 TPC 含量最高（116.65μgGAE/mg 提取物），铁皮石斛丙酮提取物（DMA）的 TFC 含量最高（116.67μgQE/mg 提取物）。铁皮石斛正己烷提取物（DMH）提取物在 800μg/ml 浓度下对 DPPH 自由基清除活性的抑制率最高（94.48%），其次是铁皮石斛乙醇提取物（DME）（94.45%）、DMA（93.71%）和 DMC（94.35%）。DMC、DMA、DMH 和 DME 的抗氧化能力（以 IC 值衡量）要低得多，分别为 42.39μg/ml、49.56μg/ml、52.68μg/ml 和 58.77μg/ml，而铁皮石斛甲醇提取物（DMM）的 IC 为 223.15μg/ml。DMM 在 800μg/ml 浓度下对 HeLa 细胞的生长抑制率最高（78.68%），而 DME 在相同浓度下对 U251 细胞的生长抑制率最高（51.95%）。DMM 对 HeLa 细胞的细胞毒性能力（IC）为 155.80μg/ml 的提取物，DME 对 U251 细胞的细胞毒性能力（IC）为 772.50μg/ml 的提取物。DME 和 DMM 中均检测到多种生物活性化合物。
结论
铁皮石斛植物提取物具有多种具有抗氧化和细胞毒性活性的生物活性化合物，这表明该植物具有潜在的药理学重要性。
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