University Institute of Biochemistry and Biotechnology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, Punjab 46000, Pakistan.
Department of Botany, Government Degree College Khanpur, Haripur 22650, Pakistan.
J Tradit Chin Med. 2019 Dec;39(6):772-779.
To investigate the phytochemicals and in vitro antioxidant, antimicrobial and cytotoxic potential of Rumex dentatus (R. dentatus) leaf extracts.
The total phenolics and flavonoids content of R. dentatus extracts were evaluated by the Folin-Ciocalteu and aluminum chloride colorimetric methods respectively. Antioxidant potential of studied plant extracts was assessed through 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity, total reducing power and total antioxidant methods. Moreover, antibacterial and antifungal capacity was also evaluated by disc diffusion method against six clinically isolated multi-drug resistant bacterial strains as well as six fungal isolates. Further, cell cytotoxicity was also evaluated through 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide assay.
Ethanol extract showed highest total phenolic [(38.9 ± 1.5) μg gallic acid equivalent/mg] and total flavonoids [(17.2 ± 1.9) μg quercetin equivalent /mg] contents. Antioxidant assays indicated that ethanol and methanol extracts possess potent antioxidant potential. Moreover, it was observed that ethanol and hexane extracts have the potential to inhibit most of the tested multi-drug resistant bacterial strains while methanol, chloroform and hexane extracts could inhibit the growth of pathogenic fungal strains successfully. Among all the studied extracts, ethanolic extract showed highest cytotoxicity against MCF-7 cell line then Hep-2 and DU-145 cell lines by MTT assay with lowest IC50 of 47.3 μg/mL.
These results suggest that R. dentatus could be a potent alternative candidate for treatment of microbial infections and for breast cancer treatment.
研究酸模叶蓼（Rumex dentatus）叶提取物的植物化学成分及其体外抗氧化、抗菌和细胞毒性。
采用福林-肖卡（Folin-Ciocalteu）和三氯化铝比色法分别测定 R. dentatus 提取物中的总酚和总黄酮含量。通过 2,2-二苯基-1-苦基肼自由基清除能力、总还原力和总抗氧化能力评估研究植物提取物的抗氧化潜力。此外，还通过圆盘扩散法评估了其对 6 株临床分离的多药耐药菌和 6 株真菌分离株的抗菌和抗真菌能力。进一步通过 3-(4,5-二甲基噻唑基-2)-2,5-二苯基四氮唑溴盐法评价细胞毒性。
乙醇提取物表现出最高的总酚含量[(38.9 ± 1.5) μg 没食子酸当量/mg]和总黄酮含量[(17.2 ± 1.9) μg 槲皮素当量/mg]。抗氧化测定表明，乙醇和甲醇提取物具有很强的抗氧化潜力。此外，观察到乙醇和正己烷提取物具有抑制大部分测试的多药耐药菌的潜力，而甲醇、氯仿和正己烷提取物能够成功抑制致病性真菌菌株的生长。在所有研究的提取物中，乙醇提取物对 MCF-7 细胞系表现出最高的细胞毒性，然后是 Hep-2 和 DU-145 细胞系，MTT 法测定的 IC50 最低为 47.3 μg/mL。
这些结果表明，R. dentatus 可能是治疗微生物感染和乳腺癌的有效替代候选药物。
