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来自Miq.叶的新型粗壮黄酮及其抗菌活性。

New robustaflavone from Miq. leave and Its antibacterial activity.

作者信息

Ambarwati Neneng Siti Silfi, Elya Berna, Malik Amarila, Omar Hanita, Hanafi Muhammad, Ahmad Islamudin

机构信息

Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, East Jakarta, Indonesia.

Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.

出版信息

J Adv Pharm Technol Res. 2022 Jan-Mar;13(1):50-55. doi: 10.4103/japtr.japtr_132_21. Epub 2022 Jan 21.

DOI:10.4103/japtr.japtr_132_21
PMID:35223441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8820349/
Abstract

Isolation and determination of antibacterial compounds from plants are essential to obtain a new antibacterial as a substitute for conventional resistant antibiotics. This study aims to isolate and identify a new robustaflavone as antibacterial activity from Miq. leave. In this study, the isolation process was carried out using column chromatography followed by preparative thin layer chromatography (TLC) based on the TLC profile. The fraction D was tested for anti-bacterial using the TLC bioautography method. The isolates obtained were then identified using 1H-NMR, 13C-NMR, distortionless enhancement by polarization transfer, heteronuclear single quantum coherence, and heteronuclear multiple bond coherence. The Activity assay of the isolate was performed using the microdilution method. A pure compound obtained the result of the separation process with eluent n-hexane: Ethyl acetate (3:2) with R 0.6. This spot follows the spot in the contact bioautographic result of fraction D, the spot with R 0.6 gives an inhibition zone. After identifying and purifying the isolate were known as Robustaflavone, this compound has activity against with a (minimum inhibitory concentration) value of 2500 ppm. Robustaflavone successfully isolated and identified from leave and its antibacterial activity.

摘要

从植物中分离和测定抗菌化合物对于获得一种新的抗菌剂以替代传统的耐药抗生素至关重要。本研究旨在从Miq.叶中分离并鉴定一种具有抗菌活性的新罗布斯塔黄酮。在本研究中，分离过程采用柱色谱法，然后根据薄层色谱（TLC）图谱进行制备型薄层色谱（TLC）。使用TLC生物自显影法对馏分D进行抗菌测试。然后使用1H-NMR、13C-NMR、极化转移无畸变增强、异核单量子相干和异核多键相干对获得的分离物进行鉴定。使用微量稀释法对分离物进行活性测定。一种纯化合物用正己烷：乙酸乙酯（3:2）洗脱剂进行分离，Rf值为0.6。该斑点与馏分D的接触生物自显影结果中的斑点一致，Rf值为0.6的斑点产生抑菌圈。在鉴定和纯化分离物后，其被确定为罗布斯塔黄酮，该化合物对……具有活性，最低抑菌浓度（MIC）值为2500 ppm。罗布斯塔黄酮成功地从叶中分离和鉴定出来，并具有抗菌活性。 （注：原文中“with a (minimum inhibitory concentration) value of 2500 ppm.”这里“with a...”后面似乎表述不完整，根据上下文推测大概意思进行了补充翻译）

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/d16d7e747d1d/JAPTR-13-50-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/90ac2dd22e2c/JAPTR-13-50-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/7ca8520504ee/JAPTR-13-50-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/3109da123ee6/JAPTR-13-50-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/901a674bc94c/JAPTR-13-50-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/4744d1192a81/JAPTR-13-50-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/393faf6f4b91/JAPTR-13-50-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/d16d7e747d1d/JAPTR-13-50-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/90ac2dd22e2c/JAPTR-13-50-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/7ca8520504ee/JAPTR-13-50-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/3109da123ee6/JAPTR-13-50-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/901a674bc94c/JAPTR-13-50-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/4744d1192a81/JAPTR-13-50-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/393faf6f4b91/JAPTR-13-50-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d7b/8820349/d16d7e747d1d/JAPTR-13-50-g007.jpg

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