Sandra A, Fyler D J, Marshall S J
Horm Metab Res. 1986 May;18(5):285-9. doi: 10.1055/s-2007-1012298.
The effect of interacting isolated rat adipocytes with small, unilammelar vesicles on insulin receptor internalization and processing was studied. Treatment of freshly isolated cells with vesicles containing phosphatidylcholine and phosphatidylserine followed by incubation in 35 mM Tris-containing buffer considerably reduced the chloroquine-induced increase in cell-associated 125I-insulin and significantly inhibited the time and insulin dependent loss of surface insulin receptors. The internal receptor pool, as measured by insulin binding to detergent solubilized adipocytes, was relatively smaller in vesicle-treated cells. Concomitant with a slower rate of receptor internalization, insulin-sensitive hexose uptake also demonstrated significantly slower kinetics of decreased response with time. These results support the conclusion that pretreatment of fat cells with phospholipid vesicles inhibits normal insulin receptor cycling.
研究了将分离的大鼠脂肪细胞与小单层囊泡相互作用对胰岛素受体内化和加工的影响。用含有磷脂酰胆碱和磷脂酰丝氨酸的囊泡处理新鲜分离的细胞，然后在含35 mM Tris的缓冲液中孵育，可显著降低氯喹诱导的细胞相关125I胰岛素增加，并显著抑制表面胰岛素受体的时间依赖性和胰岛素依赖性丢失。通过胰岛素与去污剂溶解的脂肪细胞结合来测量，囊泡处理细胞中的内化受体池相对较小。与较慢的受体内化速率相一致，胰岛素敏感的己糖摄取也显示出随时间下降反应的动力学显著减慢。这些结果支持以下结论：用磷脂囊泡预处理脂肪细胞会抑制正常的胰岛素受体循环。
