Histology Division, Basic Science Department, Faculty of Physical Therapy, Nahda University, Beni Suef, Egypt.
Molecular Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Salah Salem St, Beni-Suef, 62511, Egypt.
J Mol Histol. 2022 Jun;53(3):543-560. doi: 10.1007/s10735-022-10066-w. Epub 2022 Feb 28.
Hypothyroidism (HPO) has been linked to a higher incidence of hepatic lesions. Hesperidin (HSP) is an antioxidant, anti-adipogenic, anti-inflammatory, anti-hyperlipidemic, and anti-apoptotic agent. Therefore, the study aimed to assess the impact of carbimazole (CBZ)-induced HPO on adult albino rats' liver and explore the possible ameliorating effect of Eltroxin (ELT) and HSP. HPO was induced by CBZ (20 mg/kg/day). Rats were allocated into group I: normal control; group II: received CBZ (20 mg/kg/day) only; group III: received CBZ and HSP (200 mg/kg/day); and group IV: received CBZ and ELT (0.045 mg/kg/day). HSP and ELT attenuated dyslipidemia associated with HPO. HSP and ELT also significantly decreased elevated malondialdehyde and increased reduced glutathione levels and superoxide dismutase and catalase activities. Also, they markedly inhibited the expression of nuclear factor kappa B, inducible nitric oxide synthase, interleukin (IL)-1β, tumor necrosis factor-alpha, and alpha-smooth muscle actin. On the other hand, HSP successfully elevated nuclear factor erythroid 2-related factor 2, heme oxygenase 1, IL-37, proliferating cell nuclear antigen, and B-cell lymphoma 2 levels. Moreover, HSP decreased the activity of liver transaminases and increased total protein and albumin levels. HSP showed a protective effect on liver tissues of CBZ-treated rats. Our findings confirmed that HSP is an effective antioxidant that prevents and protects the liver from damage by CBZ. Therefore, HSP is a promising candidate for future use to minimize and alleviate HPO risks.
甲状腺功能减退症（HPO）与肝损伤的发生率升高有关。橙皮苷（HSP）是一种抗氧化剂、抗脂肪生成剂、抗炎、抗高血脂和抗凋亡剂。因此，本研究旨在评估卡比马唑（CBZ）诱导的 HPO 对成年白化大鼠肝脏的影响，并探讨依替碘酮（ELT）和 HSP 的可能改善作用。HPO 是通过 CBZ（20mg/kg/天）诱导的。将大鼠分为四组：正常对照组；仅接受 CBZ（20mg/kg/天）的组 II；接受 CBZ 和 HSP（200mg/kg/天）的组 III；接受 CBZ 和 ELT（0.045mg/kg/天）的组 IV。HSP 和 ELT 减轻了 HPO 相关的血脂异常。HSP 和 ELT 还显著降低了升高的丙二醛和增加的还原型谷胱甘肽水平以及超氧化物歧化酶和过氧化氢酶活性。此外，它们显著抑制了核因子 kappa B、诱导型一氧化氮合酶、白细胞介素（IL）-1β、肿瘤坏死因子-α和α-平滑肌肌动蛋白的表达。另一方面，HSP 成功地升高了核因子红细胞 2 相关因子 2、血红素加氧酶 1、IL-37、增殖细胞核抗原和 B 细胞淋巴瘤 2 的水平。此外，HSP 降低了肝转氨酶的活性，增加了总蛋白和白蛋白的水平。HSP 对 CBZ 处理大鼠的肝组织具有保护作用。我们的研究结果证实，HSP 是一种有效的抗氧化剂，可预防和保护肝脏免受 CBZ 的损害。因此，HSP 是未来用于最小化和减轻 HPO 风险的有前途的候选药物。
