Hegazy Walaa, Sakr Hader I, Abdul Hamid Manal, Abdelaziz Mohamed A, Salah Marwa, Abdel Rehiem Eman S, Abdel Moneim Adel
Histology Division, Basic Science Department, Faculty of Physical Therapy, Nahda University, Beni-Suef 62511, Egypt.
Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt.
Biomedicines. 2023 May 29;11(6):1570. doi: 10.3390/biomedicines11061570.
The occurrence of worsening pulmonary function has been connected to hypothyroidism (HPO). Hesperidin (HES) was suggested to have antioxidant, anti-proliferative, and anti-inflammatory potential. Our study's objective was to determine whether HES could reduce carbimazole (CBZ)-induced lung injury more effectively than Eltroxin (ELT) in adult male albino rats or not. At random, 32 rats were distributed into four groups: Group I: normal control, to induce HPO, the remaining three groups were given CBZ (20 mg/kg/day) dissolved in distilled water for 1 week. They were then split up into three groups. Group II: orally administered CBZ (20 mg/kg b.w in water/day), Group III: HES (200 mg/kg/day) dissolved in 1% carboxymethyl-cellulose + CBZ treated, and Group IV: ELT (0.045 mg/kg/day) dissolved in distilled water + CBZ treated. All treatments were delivered for 12 weeks. Blood was collected to assess thyroid-stimulating hormone (TSH) and thyroid hormones (THs). Lung injury was evaluated based on the pulmonary content of interleukin (IL)-35, IL-6, and tumor necrosis factor-alpha (TNF-α), along with the estimation of lipid peroxidation, catalase, glutathione levels, superoxide dismutase, heme oxygenase-1 (HO-1), and nuclear factor erythroid 2-related factor 2 (Nrf2). The histological, ultrastructural, and immunohistochemical study of nuclear factor Kappa-B (NF-κB) and inducible nitric oxide synthase (iNOS), together with estimating the proliferation of cells using Antigen Ki-67 in lung tissue were performed. HES and ELT primarily suppressed variable lung damage mechanisms by suppressing TSH, the NF-κB/TNF-α pathway, iNOS, lipid peroxidation, Ki-67, and inflammatory mediators. On the other hand, they improved THs, antioxidant parameters, and the Nrf2/HO-1 pathway. HES and ELT exhibited an ameliorative effect that was reflected in the histopathological, immunohistochemical, and ultrastructural results. These results indicate that HES is a pneumoprotective agent that could be a promising treatment for oxidative stress, inflammation, and proliferation.
肺功能恶化的发生与甲状腺功能减退症（HPO）有关。橙皮苷（HES）被认为具有抗氧化、抗增殖和抗炎潜力。我们研究的目的是确定在成年雄性白化大鼠中，HES是否比左旋甲状腺素（ELT）更有效地减轻卡比马唑（CBZ）诱导的肺损伤。随机将32只大鼠分为四组：第一组：正常对照组，为诱导HPO，其余三组给予溶于蒸馏水的CBZ（20mg/kg/天），持续1周。然后将它们再分为三组。第二组：口服CBZ（20mg/kg体重/天，溶于水中），第三组：溶于1%羧甲基纤维素的HES（200mg/kg/天）+CBZ处理组，第四组：溶于蒸馏水的ELT（0.045mg/kg/天）+CBZ处理组。所有处理持续12周。采集血液以评估促甲状腺激素（TSH）和甲状腺激素（THs）。基于白细胞介素（IL）-35、IL-6和肿瘤坏死因子-α（TNF-α）的肺含量评估肺损伤，同时评估脂质过氧化、过氧化氢酶、谷胱甘肽水平、超氧化物歧化酶、血红素加氧酶-1（HO-1）和核因子红细胞2相关因子2（Nrf2）。进行了核因子κB（NF-κB）和诱导型一氧化氮合酶（iNOS）的组织学、超微结构和免疫组织化学研究，并使用抗原Ki-67评估肺组织中的细胞增殖。HES和ELT主要通过抑制TSH、NF-κB/TNF-α途径、iNOS、脂质过氧化、Ki-67和炎症介质来抑制不同的肺损伤机制。另一方面，它们改善了THs、抗氧化参数和Nrf2/HO-1途径。HES和ELT表现出改善作用，这反映在组织病理学、免疫组织化学和超微结构结果中。这些结果表明，HES是一种肺保护剂，可能是治疗氧化应激、炎症和增殖的有前景的药物。
