Kaplow L S
J Histochem Cytochem. 1977 Aug;25(8):990-1000. doi: 10.1177/25.8.70465.
The application of cytochemical techniques for use with automated instruments introduces a variety of problems not encountered with traditional cytochemical stains prepared for human visualization. This is particularly true for flow-cytophotometers, which require that cells be stained in suspension, making it difficult to remove reagents once they are added to a staining mixture. It is much less a problem for pattern recognition instruments. The major difficulties relate to cell preparation, fixation, staining, controls and interpretation of instrumental data. The new cytochemical techniques or modifications of existing methods that are required for use with such instruments present a fresh challenge to cytochemists. The need for such methods is great and the rewards may be equivalent.
将细胞化学技术应用于自动化仪器会带来一系列传统细胞化学染色为人眼观察而制备时未遇到的问题。对于流式细胞光度计而言尤其如此，因为它要求细胞在悬浮液中染色，一旦试剂添加到染色混合物中就很难去除。对于模式识别仪器来说，这一问题则要小得多。主要困难涉及细胞制备、固定、染色、对照以及仪器数据的解读。与此类仪器配套使用所需的新细胞化学技术或现有方法的改进，给细胞化学家带来了新的挑战。对此类方法的需求很大，回报可能也相当可观。
