Wang Yong-Mo, Deng Jia-Xin, Zhang Jin-Xin, Jia Rui-Zong
College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China.
Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572025, Hainan, China.
Ying Yong Sheng Tai Xue Bao. 2022 Jan;33(1):119-125. doi: 10.13287/j.1001-9332.202201.028.
protein residue and accumulation in soil are two important components of the environmental safety assessment for the plantation of transgenic crops. Several rice lines with good commercial prospects have been developed in China, but it is unclear whether proteins will accumulate in soils after multiple years of rice cultivation. We planted the transgenic rice lines Minghui 63 (Huahui No. 1) and Minghui 63 for 9 years in the same field. The proteins in the rhizosphere soil were estimated with enzyme linked immunosorbent assay (ELISA) at tillering stage and on the 60th day after harvest in each year. The protein residues during the seedling, flowering and ripening stages were estimated in the first year (2012) and the last year (2020) of the experiment. In 2012, the concentration of in the rhizosphere soil of Huahui No. 1 was 1.25, 1.77, 1.97, 1.71 and 0.30 ng·g at the seedling, tillering, flowering, ripening stages and on the 60th day after harvest, respectively. In 2020, the corresponding values were 1.30, 1.69, 2.03, 1.77, and 0.43 ng·g. In 2012, the concentration of in rhizosphere soil of line Minghui 63 was 0.91, 1.52, 1.53, 1.37, and 0.12 ng·g at the seedling, tillering, flowering, ripening stages and on the 60th day after harvest, respectively. The corresponding values in 2020 were 0.95, 1.43, 1.61, 1.40, and 0.15 ng·g. Results of multi-way ANOVA showed that the effect of year was not significant, but the effects of rice variety and growth stage were significant. Our results indicated that proteins could be detected in rhizosphere soil during the growth stages of rice, but would be degraded by 60 d after harvest, and that the concentrations of proteins in the soil would not accumulate across multiple planting years.
蛋白质残留和在土壤中的积累是转基因作物种植环境安全性评估的两个重要组成部分。中国已培育出几个具有良好商业前景的水稻品系，但尚不清楚多年种植水稻后蛋白质是否会在土壤中积累。我们在同一块田地里连续9年种植转基因水稻品系明恢63（华恢1号）和明恢63。每年在分蘖期和收获后第60天，用酶联免疫吸附测定法（ELISA）测定根际土壤中的蛋白质。在试验的第一年（2012年）和最后一年（2020年）测定了幼苗期、开花期和成熟期的蛋白质残留量。2012年，华恢1号根际土壤中在幼苗期、分蘖期、开花期、成熟期和收获后第60天的浓度分别为1.25、1.77、1.97、1.71和0.30 ng·g。2020年，相应的值分别为1.30、1.69、2.03、1.77和0.43 ng·g。2012年，明恢63品系根际土壤中在幼苗期、分蘖期、开花期、成熟期和收获后第60天的浓度分别为0.91、1.52、1.53、1.37和0.12 ng·g。2020年的相应值分别为0.95、1.43、1.61、1.40和0.15 ng·g。多因素方差分析结果表明，年份效应不显著，但水稻品种和生长阶段的效应显著。我们的结果表明，在水稻生长阶段根际土壤中可检测到蛋白质，但收获后60天会降解，且土壤中蛋白质浓度不会在多年种植后积累。
