State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Haidian, Beijing, 100193, China.
Ecotoxicology. 2014 Nov;23(9):1619-28. doi: 10.1007/s10646-014-1301-z. Epub 2014 Aug 17.
As genetic engineering in plants is increasingly used to control agricultural pests, it is important to determine whether such transgenic plants adversely affect non-target organisms within and around cultivated fields. The cry1Ab/1Ac fusion gene from Bacillus thuringiensis (Bt) has insecticidal activity and has been introduced into rice line Minghui 63 (MH63). We evaluated the effect of transgenic cry1Ab/1Ac rice (Huahui 1, HH1) on paddy frogs by comparing HH1 and MH63 rice paddies with and without pesticide treatment. The density of tadpoles in rice fields was surveyed at regular intervals, and Cry1Ab/1Ac protein levels were determined in tissues of tadpoles and froglets collected from the paddy fields. In addition, Rana nigromaculata froglets were raised in purse nets placed within these experimental plots. The survival, body weight, feeding habits, and histological characteristics of the digestive tract of these froglets were analyzed. We found that the tadpole density was significantly decreased immediately after pesticide application, and the weight of R. nigromaculata froglets of pesticide groups was significantly reduced compared with no pesticide treatment, but we found no differences between Bt and non-Bt rice groups. Moreover, no Cry1Ab/1Ac protein was detected in tissue samples collected from 192 tadpoles and froglets representing all four experimental groups. In addition, R. nigromaculata froglets raised in purse seines fed primarily on stem borer and non-target insects, and showed no obvious abnormality in the microstructure of their digestive tracts. Based on these results, we conclude that cultivation of transgenic cry1Ab/1Ac rice does not adversely affect paddy frogs.
随着植物基因工程技术越来越多地用于控制农业害虫，确定此类转基因植物是否会对农田内和周围的非目标生物产生不利影响变得尤为重要。苏云金芽孢杆菌（Bt）的 cry1Ab/1Ac 融合基因具有杀虫活性，已被导入水稻品系明恢 63（MH63）。我们通过比较转 cry1Ab/1Ac 大米（华恢 1 号，HH1）和 MH63 稻田有无农药处理，评估了转 cry1Ab/1Ac 大米对稻田青蛙的影响。定期调查稻田中蝌蚪的密度，并从稻田中收集的蝌蚪和幼蛙组织中测定 Cry1Ab/1Ac 蛋白水平。此外，还在这些实验田中放置网兜，饲养 Rana nigromaculata 幼蛙。分析了这些幼蛙的存活、体重、摄食习性和消化道的组织学特征。我们发现，施药后立即降低了蝌蚪密度，与未施药处理相比，施药组 R. nigromaculata 幼蛙的体重明显减轻，但 Bt 和非 Bt 水稻组之间没有差异。此外，在来自所有四个实验组的 192 只蝌蚪和幼蛙的组织样本中均未检测到 Cry1Ab/1Ac 蛋白。此外，在网兜里饲养的 R. nigromaculata 幼蛙主要以螟虫和非靶标昆虫为食，其消化道的微观结构没有明显异常。基于这些结果，我们得出结论，种植转 cry1Ab/1Ac 大米不会对稻田青蛙造成不利影响。
