Lu Chang-Ming, Li Xiang, Xu Ming-Kai, Li Xin-Yu, Li Xu, Gu Wu, Guo Qiu-Cui, Zhang Hui-Wen
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Ying Yong Sheng Tai Xue Bao. 2022 Jan;33(1):229-238. doi: 10.13287/j.1001-9332.202201.032.
In this study, triazine-degrading strain SB5 was isolated and screened from the activated sludge contaminated with atrazine by enrichment culture technology. Based on its morphology and 16S rRNA gene analysis, strain SB5 was initially identified as sp. It contained the atrazine-degrading genes , and . The addition of glucose, sucrose, sodium citrate, yeast extract and peptone to the culture medium significantly increased the biomass and atrazine degradation efficiency of strain SB5. The addition of (NH)SO and NHCl inhibited the biomass of strain SB5, but did not affect its degradation efficiency for atrazine. The addition of starch did not affect the biomass of strain SB5, but significantly inhibited its degradation for atrazine. Strain SB5 showed good atrazine tolerance and atrazine degradation ability in the temperature range of 4-42 ℃, initial pH of 4-10 and initial concentration of 50-1000 mg·L. Using 100 mg·L atrazine as the sole carbon source, the strain SB5 degraded 100% of atrazine within 36 h under the optimal conditions of 37 ℃ and initial pH 8.0. The results of degradation spectrum analysis showed that strain SB5 had a good degradation effect on the six triazine herbicides (simazine, terbuthylazine, propazine, cyanazine, ametryn and prometryn) at an initial concentration of 100 mg·L, and the degradation rates were 86.4%, 92%, 98.6%, 95.6%, 100% and 99.2% after 48 h of incubation, respectively. The results demonstrated that SB5 was an efficient and broad-spectrum degradation strain. The strain SB5 further enriched the strain resources for atrazine biodegradation, and its high-efficient and broad-spectrum degradation characteristics for triazine herbicides showed a potential application value in the development of bioremediation technology for the pollution of triazine herbicides.
在本研究中，通过富集培养技术从被阿特拉津污染的活性污泥中分离筛选出三嗪降解菌株SB5。基于其形态学和16S rRNA基因分析，菌株SB5初步被鉴定为 属。它含有阿特拉津降解基因 、 和 。向培养基中添加葡萄糖、蔗糖、柠檬酸钠、酵母提取物和蛋白胨显著提高了菌株SB5的生物量和阿特拉津降解效率。添加硫酸铵和氯化铵抑制了菌株SB5的生物量，但不影响其对阿特拉津的降解效率。添加淀粉不影响菌株SB5的生物量，但显著抑制其对阿特拉津的降解。菌株SB5在4-42℃温度范围、初始pH值4-10和初始浓度50-1000 mg·L条件下表现出良好的阿特拉津耐受性和阿特拉津降解能力。以100 mg·L阿特拉津作为唯一碳源，在37℃和初始pH 8.0的最佳条件下，菌株SB5在36 h内降解了100%的阿特拉津。降解谱分析结果表明，菌株SB5对初始浓度为100 mg·L的六种三嗪类除草剂（西玛津、特丁津、扑灭津、氰草津、莠灭净和扑草净）具有良好的降解效果，培养48 h后的降解率分别为86.4%、92%、98.6%、95.6%、100%和99.2%。结果表明SB5是一种高效广谱降解菌株。菌株SB5进一步丰富了阿特拉津生物降解的菌株资源，其对三嗪类除草剂的高效广谱降解特性在三嗪类除草剂污染生物修复技术开发中显示出潜在应用价值。
