宿主辅助将模型药物递送至基因组DNA：来自超快光谱学和计算机模拟研究的关键信息。

Host-Assisted Delivery of a Model Drug to Genomic DNA: Key Information from Ultrafast Spectroscopy and in silico Study.

作者信息

Ghosh Ria, Singh Soumendra, Mukherjee Dipanjan, Mondal Susmita, Das Monojit, Pal Uttam, Adhikari Aniruddha, Bhushan Aman, Bose Surajit, Bhattacharyya Siddharth Sankar, Pal Debasish, Saha-Dasgupta Tanusri, Bhattacharyya Maitree, Bhattacharyya Debasis, Mallick Asim Kumar, Das Ranjan, Pal Samir Kumar

机构信息

Department of Chemical Biological and Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences, Block JD, Sector 3, Salt lake, Kolkata 700106, India.

Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Rd, Ballygunge, Kolkata 700019, India.

出版信息

Chembiochem. 2022 May 4;23(9):e202200109. doi: 10.1002/cbic.202200109. Epub 2022 Mar 11.

DOI:10.1002/cbic.202200109

PMID:35225409

Abstract

Drug delivery to a target without adverse effects is one of the major criteria for clinical use. Herein, we have made an attempt to explore the delivery efficacy of SDS surfactant in a monomer and micellar stage during the delivery of the model drug, Toluidine Blue (TB) from the micellar cavity to DNA. Molecular recognition of pre-micellar SDS encapsulated TB with DNA occurs at a rate constant of k ∼652 s . However, no significant release of encapsulated TB at micellar concentration was observed within the experimental time frame. This originated from the higher binding affinity of TB towards the nano-cavity of SDS at micellar concentration which does not allow the delivery of TB from the nano-cavity of SDS micelles to DNA. Thus, molecular recognition controls the extent of DNA recognition by TB which in turn modulates the rate of delivery of TB from SDS in a concentration-dependent manner.

摘要

在无不良反应的情况下将药物输送到靶点是临床应用的主要标准之一。在此，我们尝试探索十二烷基硫酸钠（SDS）表面活性剂在单体和胶束阶段对模型药物甲苯胺蓝（TB）从胶束腔向DNA递送过程中的递送效果。预胶束SDS包裹的TB与DNA的分子识别以速率常数k ∼652 s 发生。然而，在实验时间范围内，未观察到胶束浓度下包裹的TB有明显释放。这源于胶束浓度下TB对SDS纳米腔的较高结合亲和力，这使得TB无法从SDS胶束的纳米腔递送至DNA。因此，分子识别控制着TB对DNA的识别程度，进而以浓度依赖的方式调节TB从SDS的递送速率。