Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia Governorate, Egypt.
Histology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia Governorate, Egypt.
Appl Physiol Nutr Metab. 2022 Mar;47(3):296-308. doi: 10.1139/apnm-2021-0133. Epub 2022 Feb 28.
This study aimed to investigate the possible ameliorative effects of co-supplementation with Mg and treadmill exercise on memory deficit in aged rats. Fifty male albino rats (10 young and 40 aged rats) were divided into 5 groups (10 rats/group): young, aged sedentary, aged exercised, aged Mg-supplemented, and aged exercised and Mg-supplemented. Memory was assessed using the Y-maze and novel object recognition tests. Plasma samples were collected for measurement of C-reactive protein (CRP). Subsequently, brain malondialdehyde and catalase levels were measured. Histological and immunohistochemical analyses of the hippocampi were performed. Our results showed impaired memory in aged sedentary rats, with significantly elevated plasma CRP and brain malondialdehyde levels and decreased brain catalase. The hippocampus of aged sedentary rats showed cellular degeneration, downregulation of synaptophysin (SYP) and proliferating cell nuclear antigen (PCNA), and upregulation of glial fibrillary acidic protein (GFAP) and caspase-3. Mg supplementation and/or treadmill exercise significantly improved memory tests in aged rats, which could be explained by the upregulation of hippocampal SYP and PCNA expression and downregulation of GFAP and caspase-3 expression with antioxidant and anti-inflammatory mechanisms. The combined therapy had a better effect than both treatments alone, confirming the role of Mg supplementation with physical exercise in enhancing age-related memory deficit. Magnesium supplementation with treadmill exercise improves memory deficit in aged rats. The possible mechanisms are upregulation of the hippocampal synaptophysin and PCNA, downregulation of GFAP and caspase-3, the antioxidant and anti-inflammatory mechanisms.
本研究旨在探讨镁联合跑步机运动对老年大鼠记忆缺陷的可能改善作用。将 50 只雄性白化大鼠（10 只幼鼠和 40 只老年鼠）分为 5 组（每组 10 只）：幼鼠组、老年安静组、老年运动组、老年镁补充组和老年运动加镁补充组。使用 Y 迷宫和新物体识别测试评估记忆。采集血浆样本，测量 C 反应蛋白（CRP）。随后，测量大脑丙二醛和过氧化氢酶水平。对海马进行组织学和免疫组织化学分析。我们的结果表明，老年安静组大鼠的记忆受损，血浆 CRP 和大脑丙二醛水平显著升高，大脑过氧化氢酶水平降低。老年安静组大鼠的海马出现细胞变性，突触素（SYP）和增殖细胞核抗原（PCNA）表达下调，胶质纤维酸性蛋白（GFAP）和半胱氨酸天冬氨酸蛋白酶-3（caspase-3）表达上调。镁补充和/或跑步机运动可显著改善老年大鼠的记忆测试，这可以通过海马 SYP 和 PCNA 表达上调以及 GFAP 和 caspase-3 表达下调以及抗氧化和抗炎机制来解释。联合治疗比单独两种治疗的效果更好，证实了镁补充与体育锻炼在增强与年龄相关的记忆缺陷方面的作用。镁补充联合跑步机运动可改善老年大鼠的记忆缺陷。可能的机制是上调海马 SYP 和 PCNA，下调 GFAP 和 caspase-3，以及抗氧化和抗炎机制。
