Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
School of Food Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
J Agric Food Chem. 2022 Mar 9;70(9):2839-2850. doi: 10.1021/acs.jafc.1c07361. Epub 2022 Feb 28.
Transferrins are multifunctional proteins, but their role in the interaction of rice and brown planthopper (BPH) remains unclear. In this study, the full-length cDNA of transferrin genes , , and was cloned. Reverse transcription quantitative polymerase chain reaction showed that the expressions of and were significantly suppressed in BPH reared on the resistant rice R1 by 68.0 and 86.7%, respectively, compared with that on the susceptible S9. The survival rate decreased to 3.3% for dsNlTsf3-treated nymphs, to 58.9% for dsNlTsf1, and to 56.7% for dsNlTsf2 on day 11. RNAi of against females largely reduced the number of eggs by 99.4%, and it decreased by 48.6% for dsNlTsf1 but did not significantly decrease for dsNlTsf2. Collectively, , , and are essential for the survival and fecundity of BPH and are differentially involved in the interaction between rice and BPH. Therefore, and may be used as targets to control BPH.
转铁蛋白是多功能蛋白，但它们在水稻与褐飞虱（BPH）相互作用中的作用尚不清楚。本研究克隆了转铁蛋白基因、和全长 cDNA。反转录定量聚合酶链式反应显示，与在感虫 S9 上相比，在抗虫稻 R1 上饲养的 BPH 中 和 的表达分别显著下调了 68.0%和 86.7%。dsNlTsf3 处理的若虫存活率在第 11 天降至 3.3%，dsNlTsf1 降至 58.9%，dsNlTsf2 降至 56.7%。针对雌虫的 RNAi 使卵的数量减少了 99.4%，dsNlTsf1 减少了 48.6%，但 dsNlTsf2 没有显著减少。综上所述，、和是 BPH 生存和繁殖所必需的，并且它们在水稻与 BPH 的相互作用中具有不同的作用。因此，和可能被用作防治 BPH 的靶标。
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