Yang Zhifan, Zhang Futie, He Qing, He Guangcun
Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
Arch Insect Biochem Physiol. 2005 Jun;59(2):59-66. doi: 10.1002/arch.20055.
To investigate the molecular response of brown planthopper, Nilaparvata lugens (BPH) to BPH-resistant rice plants, we isolated cDNA fragments of the genes encoding for carboxylesterase (CAR), trypsin (TRY), cytochrome P450 monooxygenase (P450), NADH-quinone oxidoreductase (NQO), acetylcholinesterase (ACE), and Glutathione S-transferase (GST). Expression profiles of the genes were monitored on fourth instar nymphs feeding on rice varieties with different resistance levels. Northern blot hybridization showed that, compared with BPH reared on susceptible rice TN1, expression of the genes for P450 and CAR was apparently up-regulated and TRY mRNA decreased in BPH feeding on a highly resistant rice line B5 and a moderately resistant rice variety MH63, respectively. Two transcripts of GST increased in BPH feeding on B5; but in BPH feeding on MH63, this gene was inducible and its expression reached a maximum level at 24 h, and then decreased slightly. The expression of NQO gene was enhanced in BPH on B5 plants but showed a constant expression in BPH on MH63 plants. No difference in ACE gene expression among BPH on different rice plants was detected by the RT-PCR method. The results suggest these genes may play important roles in the defense response of BPH to resistant rice.
为了研究褐飞虱（Nilaparvata lugens，BPH）对抗褐飞虱水稻植株的分子响应，我们分离了编码羧酸酯酶（CAR）、胰蛋白酶（TRY）、细胞色素P450单加氧酶（P450）、NADH-醌氧化还原酶（NQO）、乙酰胆碱酯酶（ACE）和谷胱甘肽S-转移酶（GST）的基因的cDNA片段。在取食不同抗性水平水稻品种的四龄若虫上监测这些基因的表达谱。Northern杂交显示，与在感虫水稻TN1上饲养的褐飞虱相比，取食高抗水稻品系B5和中抗水稻品种MH63的褐飞虱中，P450和CAR基因的表达明显上调，而TRY mRNA分别下降。取食B5的褐飞虱中GST的两种转录本增加；但在取食MH63的褐飞虱中，该基因是可诱导的，其表达在24小时达到最高水平，然后略有下降。NQO基因在取食B5植株的褐飞虱中表达增强，但在取食MH63植株的褐飞虱中表达恒定。通过RT-PCR方法未检测到不同水稻植株上的褐飞虱之间ACE基因表达的差异。结果表明这些基因可能在褐飞虱对抗性水稻的防御反应中发挥重要作用。
