University of Belgrade, Faculty of Medicine and BelPrime Clinic, Belgrade, Serbia.
Laboratory of Cell Biology and Developmental Pathology FSBSI, Moscow, Russia.
Exp Dermatol. 2022 Jun;31(6):936-942. doi: 10.1111/exd.14548. Epub 2022 Mar 19.
Hair folliculogenesis and hair growth mediated by the secretory properties of white adipocytes may pave the way for the adipose-derived (AD) regenerative therapy for androgenetic alopecia (AGA). Quantitative and qualitative secretome profiling of AD stem cells (ADSCs) from different zones of hair growth in patients with AGA were analysed. 1-mm punch samples of adipose tissue associated with hair follicles, of three scalp areas (balding, non-balding and transition zones) and one periumbilical sample, were used for ADCS isolation. The ADCS secretome was analysed in conditioned media using a 41plex assay. Among the thirty-five signalling proteins analysed, the levels of VEGF, EGF, IL-6, Eotaxin, MCP-3, IFNγ-inducible protein-10 and MIP-1α were higher in the balding zone compared with the non-balding and periumbilical zones. In contrast, MCP-1 was the lowest in the balding zone in comparison with the other zones. The observed differences in the secretome suggest crosstalk between angiogenic and inflammatory processes underlying AGA aetiology and may prove relevant in both the diagnosis of AGA and the application of ADSC secretome for AGA treatment.
脂肪细胞的分泌特性介导的毛囊发生和毛发生长可能为脂肪来源（AD）再生疗法治疗雄激素性脱发（AGA）铺平道路。对AGA 患者不同生长区 AD 干细胞（ADSC）的定量和定性分泌组进行了分析。使用 1mm 打孔器从三个头皮区域（脱发、非脱发和过渡区）和一个脐周区采集与毛囊相关的脂肪组织样本，用于 ADSC 分离。使用 41plex 检测法分析 ADCS 条件培养基中的 ADCS 分泌组。在所分析的 35 种信号蛋白中，与非脱发和脐周区相比，脱发区的 VEGF、EGF、IL-6、Eotaxin、MCP-3、IFNγ 诱导蛋白-10 和 MIP-1α 水平更高。相反，与其他区域相比，MCP-1 在脱发区的水平最低。分泌组中观察到的差异表明，AGA 发病机制中的血管生成和炎症过程之间存在串扰，这可能与 AGA 的诊断和 ADSC 分泌组用于 AGA 治疗都相关。
