Wang Zhiwei, Pan Ni, Yan Jiahao, Wan Jian, Wan Cuihong
School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei 430079, People's Republic of China.
J Proteome Res. 2022 Apr 1;21(4):1114-1123. doi: 10.1021/acs.jproteome.2c00004. Epub 2022 Feb 28.
Short open reading frame-encoded peptides (SEPs) are microproteins with less than 100 amino acids that play an essential role in the growth and development of organisms. There are plenty of short open reading frames in that potentially code polypeptides. We chose 11 time points during the life cycle of to investigate microproteins, particularly those related to development. Finally, we identified a total of 410 microproteins, of which 27 were noncoding RNA-encoded proteins. Of the 410 microproteins, 74 were expressed in all stages from embryo to adults, whereas 300 microproteins were only found in one or two time points. Approximately, one-third of the microproteins were not reported previously and 44 were obtained from sequencing, validated by synthetic peptides. These microproteins are related to the main bioprocesses of growth and development, such as multicellular organism reproduction, postmating behavior, and oviposition. Over half of the microproteins have predicted functional domains and are conserved across species, suggesting that these microproteins have critical functions in fly development. This work enriches the proteome and provides a significant data resource for growth and development research.
短开放阅读框编码肽（SEPs）是由少于100个氨基酸组成的微蛋白，在生物体的生长发育中起着至关重要的作用。基因组中存在大量可能编码多肽的短开放阅读框。我们在[具体物种]的生命周期中选取了11个时间点来研究微蛋白，特别是那些与发育相关的微蛋白。最终，我们总共鉴定出410个微蛋白，其中27个是非编码RNA编码的蛋白。在这410个微蛋白中，74个在从胚胎到成虫的所有阶段均有表达，而300个微蛋白仅在一两个时间点被发现。大约三分之一的微蛋白此前未被报道，44个是通过[具体物种]测序获得的，并经合成肽验证。这些微蛋白与生长发育的主要生物过程相关，如多细胞生物繁殖、交配后行为和产卵。超过一半的微蛋白具有预测的功能结构域且在物种间保守，这表明这些微蛋白在果蝇发育中具有关键功能。这项工作丰富了[具体物种]的蛋白质组，为生长发育研究提供了重要的数据资源。
