Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei, 050024, PR China.
Department of Pathogenic Biology, College of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei, 050017, PR China.
Exp Parasitol. 2022 Apr;235:108232. doi: 10.1016/j.exppara.2022.108232. Epub 2022 Feb 25.
Babesia is a protozoan that mainly parasitizes mammalian red blood cells. It causes damage to multiple organs of the host, even threatening the life of the host when the infection is severe. This study found that the mouse kidney was injured after Babesia infection, leading to changes such as ischaemia and an abnormal morphology of renal and epithelial cells. Serum tests showed that indices reflecting renal abnormalities (including serum creatinine, uric acid, and bilirubin) appeared to be abnormal. To further explore the molecular mechanism underlying kidney injury and self-healing in infected hosts, we employed a data-independent acquisition (DIA) proteomics method to investigate large-scale B. microti infection-induced changes in protein expression and phosphorylation in mouse kidneys. This study identified and analysed the reasons for the obvious changes in kidney injury-related proteins, repair-related proteins, immune-related proteins, and lipid metabolism-related proteins. The results provide a strong theoretical basis for effective treatments of the kidney disease caused by Babesia infection.
巴贝虫是一种主要寄生在哺乳动物红细胞内的原生动物。它会对宿主的多个器官造成损伤，当感染严重时，甚至会威胁到宿主的生命。本研究发现巴贝虫感染后小鼠肾脏受损，导致肾脏和上皮细胞发生缺血和形态异常等变化。血清学检测显示，反映肾脏异常的指标（包括血清肌酐、尿酸和胆红素）似乎出现异常。为了进一步探究感染宿主肾脏损伤和自我修复的分子机制，我们采用了一种非依赖性采集（DIA）蛋白质组学方法，研究了 B. microti 大规模感染诱导的小鼠肾脏中蛋白质表达和磷酸化的变化。本研究鉴定和分析了与肾脏损伤相关蛋白、修复相关蛋白、免疫相关蛋白和脂质代谢相关蛋白明显变化的原因。研究结果为有效治疗巴贝虫感染引起的肾脏疾病提供了强有力的理论依据。
