Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province, School of Environmental and Natural Resources, Zhejiang University of Science and Technology, Hangzhou, 310023, China.
Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province, School of Environmental and Natural Resources, Zhejiang University of Science and Technology, Hangzhou, 310023, China.
Ecotoxicol Environ Saf. 2020 Jul 15;198:110665. doi: 10.1016/j.ecoenv.2020.110665. Epub 2020 Apr 27.
Due to the wide use of plastic products and the releasability of plasticizer into surrounding environment, the hazards, residues and effects of phthalic acid esters (PAEs) in ecosystems have been paid more and more attention. Little information is available about the effects of PAEs on the normal wastewater treatment, although the distribution of PAEs in soil and other ecosystems is closely related to the discharge of sewage. In this study, the effects of high concentrations of di-n-butyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP) on pollutant removal and the microbial community during landfill leachate treatment was investigated. After domestication, the activated sludge was used in the co-treatment of landfill leachate and simulated domestic wastewater. We verified that this process reduced the toxicity of landfill leachate. However, high concentrations of added DBP and DEHP were removed first, while the removal of these pollutants from raw landfill leachate was limited. The results of high-throughput sequencing revealed that the bacterial diversity was diminished and the microbial community structure was significantly affected by the addition of DBP and DEHP. The DBP and DEHP samples had 79.05% and 82.25% operational taxonomic units (OTU), respectively, in common with the raw activated sludge. Many genera of PAE-degrading bacteria that had no significant evolutionary relationship were found in the raw activated sludge. And the widespread presence of PAE-degrading bacteria could effectively keep the concentrations of PAEs low during the wastewater treatment.
由于塑料制品的广泛使用以及增塑剂向周围环境的释放，邻苯二甲酸酯（PAEs）在生态系统中的危害、残留和影响越来越受到关注。尽管 PAEs 在土壤和其他生态系统中的分布与污水排放密切相关，但关于 PAEs 对正常废水处理的影响的信息却很少。在这项研究中，研究了高浓度邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）对垃圾渗滤液处理过程中污染物去除和微生物群落的影响。驯化后，采用活性污泥协同处理垃圾渗滤液和模拟生活污水。我们验证了该过程降低了垃圾渗滤液的毒性。然而，高浓度添加的 DBP 和 DEHP 首先被去除，而从原始垃圾渗滤液中去除这些污染物的效果有限。高通量测序结果表明，DBP 和 DEHP 的添加显著降低了细菌多样性，并影响了微生物群落结构。DBP 和 DEHP 样品与原始活性污泥分别有 79.05%和 82.25%的操作分类单元（OTU）具有共同性。在原始活性污泥中发现了许多与 PAE 降解菌没有明显进化关系的 PAE 降解菌属。而且，PAE 降解菌的广泛存在可以有效地将 PAEs 的浓度保持在废水处理过程中的较低水平。
