Department of Bioinformatics and Biotechnology, Faculty of Life Sciences, Government College University Faisalabad, Pakistan.
Department of Microbiology, Faculty of Life Sciences, Government College University Faisalabad, Pakistan.
Pak J Pharm Sci. 2022 Jan;35(1(Supplementary)):203-208.
Carbapenem-resistant Acinetobacter baumannii (CRAB) produce resistance to various classes of antibiotics and left limited options for treatment. This study was designed to determine antibacterial activity of AgNPs against CRAB. Total 100 A. baumannii were collected from a tertiary care hospital, Lahore. Isolates were subcultured on blood and MacConkey agar. Preliminary identification was carried out by morphological and biochemical tests. Antibiogram was done by Kirby-Bauer disc diffusion method. Antibacterial activity of AgNPs was performed by agar well diffusion method, while minimum inhibitory concentration and minimum bactericidal concentration were determined by micro broth dilution assay. Of 100 A. baumannii, 24 were confirmed as carbapenem-resistant. These isolates were mainly recovered from tracheal secretion (8; 33%), CSF (5; 20.8%), and urine (4; 16.8%). Antibacterial activity of AgNPs revealed a maximum zone of inhibition, 22mm at 50mg/mL and 18mm at 40mg/mL by agar well diffusion method. MIC of AgNPs determined that 14 CRAB were inhibited at 12.5mg/mL and 7 at 25mg/mL. However, MBC revealed that 13 CRAB were killed at 25mg/mL and 7 at 50mg/mL. This study concluded that most of the CRAB were inhibited and killed at 12.5mg/mL and 25mg/mL, respectively. AgNPs can be used as an alternative therapeutic agent followed by their pharmacokinetics and pharmacognosy.
耐碳青霉烯鲍曼不动杆菌 (CRAB) 对各种类别的抗生素产生耐药性，治疗选择有限。本研究旨在确定 AgNPs 对 CRAB 的抗菌活性。从拉合尔的一家三级保健医院收集了总共 100 株鲍曼不动杆菌。将分离物在血液和 MacConkey 琼脂上进行传代培养。通过形态学和生化试验进行初步鉴定。通过 Kirby-Bauer 纸片扩散法进行抗生素敏感性试验。通过琼脂孔扩散法进行 AgNPs 的抗菌活性，通过微量肉汤稀释法测定最小抑菌浓度和最小杀菌浓度。在 100 株鲍曼不动杆菌中，24 株被确认为耐碳青霉烯。这些分离株主要从气管分泌物（8；33%）、CSF（5；20.8%）和尿液（4；16.8%）中回收。AgNPs 的抗菌活性通过琼脂孔扩散法显示出最大的抑制区，在 50mg/mL 时为 22mm，在 40mg/mL 时为 18mm。AgNPs 的 MIC 测定结果表明，14 株 CRAB 在 12.5mg/mL 时被抑制，7 株在 25mg/mL 时被抑制。然而，MBC 显示 13 株 CRAB 在 25mg/mL 时被杀灭，7 株在 50mg/mL 时被杀灭。本研究得出结论，大多数 CRAB 在 12.5mg/mL 和 25mg/mL 时被抑制和杀灭。AgNPs 可以作为一种替代治疗药物，随后进行药代动力学和药理学研究。
