Siddiqui Fatima, Zahra Nureen, Amjad Muhammad Faisal, Zeshan Basit, Alam Rabail, Jaffar Hafiza Madiha, Aziz Tariq, Alshehri Fatma, Al-Asmari Fahad, Al-Joufi Fakhria A, Alwethaynani Maher S, Baothman Bandar K
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, 54000, Punjab, Pakistan.
Department of Biotechnology, GC University, Lahore, 54000, Punjab, Pakistan.
Sci Rep. 2025 May 20;15(1):17465. doi: 10.1038/s41598-025-01639-2.
Multidrug resistant Acinetobacter baumannii is declared as crucial level precedence pathogen by World Health Organization that needs new and upgraded antibiotics for better treatment. Against a vast extent of microbes, many silver nanoparticles have displayed anti-microbial activity because of their numerous methods of antimicrobial actions. This study was aimed to isolate and characterize the Acinetobacter baumannii by using standard microbiological technique and to analyze the anti-inflammatory and antagonistic effect of PB capped AgNPs in isolated A. baumannii. In antagonistic activity, PB capped AgNPs showed antagonistic effect in 8 out of 20 isolates tested and PB when combined with AgNPs showed antagonistic effect in all isolates tested. In in-vitro anti-inflammatory egg albumin assay, PB capped AgNPs gave inhibition rate of 96% at 12.5 µg, 93% at 25 µg, 82% at 50 µg and 62% at 100 µg whereas in Polymyxin B combined AgNPs it gave inhibition rate of 97% at the dose of 50 µg. In-silico analysis also showed that PB in combination with tri-sodium citrate gave good binding energy than tri-sodium citrate and Polymyxin used alone. Hence, this study represented that silver nanoparticles when used in combination proved to be a good alternative in the treatment of MDR Acinetobacter baumannii.
多重耐药鲍曼不动杆菌被世界卫生组织列为关键优先级别病原体，需要新型和升级的抗生素来进行更好的治疗。由于其多种抗菌作用方式，许多银纳米颗粒对多种微生物都显示出抗菌活性。本研究旨在通过标准微生物技术分离和鉴定鲍曼不动杆菌，并分析聚丁二炔包覆的银纳米颗粒（PB capped AgNPs）对分离出的鲍曼不动杆菌的抗炎和拮抗作用。在拮抗活性方面，在测试的20株菌株中，聚丁二炔包覆的银纳米颗粒对8株显示出拮抗作用，而聚丁二炔与银纳米颗粒联合使用时对所有测试菌株均显示出拮抗作用。在体外抗炎蛋清蛋白测定中，聚丁二炔包覆的银纳米颗粒在12.5微克时抑制率为96%，25微克时为93%，50微克时为82%，100微克时为62%，而在多粘菌素B联合银纳米颗粒中，在50微克剂量时抑制率为97%。计算机模拟分析还表明，聚丁二炔与柠檬酸三钠联合使用时的结合能比单独使用柠檬酸三钠和多粘菌素时更好。因此，本研究表明，银纳米颗粒联合使用被证明是治疗多重耐药鲍曼不动杆菌的一种良好替代方法。
