Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650021, China.
Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, 610041, China.
Sci Rep. 2022 Feb 28;12(1):3264. doi: 10.1038/s41598-022-07285-2.
Despite many studies on the influence of cropping practices on soil microbial community structure, little is known about ecological patterns of rare and abundant microbial communities in response to different tobacco cropping systems. Here, using the high-throughput sequencing technique, we investigated the impacts of two different cropping systems on soil biochemical properties and the microbial community composition of abundant and rare taxa and its driving factors in continuous and rotational tobacco cropping systems in the mountain lands of Yunnan, China. Our results showed that distinct co-occurrence patterns and driving forces for abundant and rare taxa across the different cropping systems. The abundant taxa were mainly constrained by stochastic processes in both cropping systems. In contrast, rare taxa in continuous cropping fields were mainly influenced by environmental perturbation (cropping practice), while governed by deterministic processes under rotational cropping. The α-diversity indices of rare taxa tended to be higher than those of the abundant ones in the two cropping systems. Furthermore, the network topologies of rare taxa were more complex than those of the abundant taxa in the two cropping systems. These results highlight that rare taxa rather than abundant ones play important roles in maintaining ecosystem diversity and sustaining the stability of ecosystem functions, especially in continuous cropping systems.
尽管有许多关于耕作方式对土壤微生物群落结构影响的研究，但对于不同烟草种植系统中稀有和丰富微生物群落的生态模式知之甚少。在这里，我们使用高通量测序技术，研究了两种不同的种植系统对土壤生化特性以及丰富和稀有分类群的微生物群落组成的影响，以及它们在中国云南山地连续和轮作烟草种植系统中的驱动因素。研究结果表明，不同种植系统中丰富和稀有分类群的共现模式和驱动因素明显不同。丰富的分类群主要受到两个种植系统中随机过程的限制。相比之下，连续种植田中的稀有分类群主要受环境干扰（耕作实践）的影响，而在轮作种植系统中则受确定性过程的控制。稀有分类群的α多样性指数在两种种植系统中均高于丰富分类群。此外，稀有分类群的网络拓扑结构比两种种植系统中丰富分类群的网络拓扑结构更复杂。这些结果表明，稀有分类群而非丰富分类群在维持生态系统多样性和维持生态系统功能稳定性方面发挥着重要作用，特别是在连续种植系统中。
