State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China.
School of Marine Sciences, Ningbo University, Ningbo, 315211, China.
Environ Microbiol. 2020 May;22(5):1707-1719. doi: 10.1111/1462-2920.14820. Epub 2019 Dec 11.
Diatom blooms can significantly influence the dynamics of microbial communities, yet little is known about the interaction and assembly mechanisms of abundant and rare taxa during bloom process. Here, using 16S rRNA gene amplicon sequencing, we investigated the co-occurrence patterns and assembly processes of abundant and rare microbial communities during an early spring diatom bloom in Xiangshan bay. Our results showed that α-diversity indices in the rare subcommunity (RS) were significantly higher than those in the abundant and common subcommunities. β-Diversity of the RS was the highest among three subcommunities, and the variation of β-diversity in the three subcommunities was mainly induced by species turnover, which was also the highest in the RS. The assembly of microbial communities was mainly driven by the neutral processes, but the roles of neutral processes might differ in each subcommunity. Co-occurrence network analysis revealed that abundant and common operational taxonomic units were more often located in central positions within the network. Most of the modules in the network were specific to a particular bloom stage, owing to the succession of Skeletonema costatum. Overall, these findings expand current understanding of the microbial interaction and assembly mechanisms in marine environment suffering harmful algal bloom disturbance.
硅藻水华可以显著影响微生物群落的动态，但对于富营养和稀有分类群在水华过程中的相互作用和组装机制知之甚少。在这里，我们使用 16S rRNA 基因扩增子测序，研究了象山湾春季早期硅藻水华过程中丰富和稀有微生物群落的共现模式和组装过程。我们的结果表明，稀有亚群（RS）的α多样性指数明显高于丰富和常见亚群。RS 的β多样性在三个亚群中最高，三个亚群的β多样性变化主要是由物种更替引起的，而在 RS 中这一变化最高。微生物群落的组装主要受中性过程驱动，但中性过程在每个亚群中的作用可能不同。共现网络分析表明，丰富和常见的操作分类单元在网络中更经常处于中心位置。由于拟菱形藻的演替，网络中的大多数模块都特定于特定的水华阶段。总的来说，这些发现扩展了我们对海洋环境中有害藻华干扰下微生物相互作用和组装机制的现有认识。
