基于铁基固体脂质纳米粒的新型磁共振结肠成像技术的研发。

Development of a Novel MR Colonography via Iron-Based Solid Lipid Nanoparticles.

机构信息

Department of Radiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, People's Republic of China.

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, People's Republic of China.

出版信息

Int J Nanomedicine. 2022 Feb 22;17:821-836. doi: 10.2147/IJN.S347498. eCollection 2022.

DOI:10.2147/IJN.S347498

PMID:35228799

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8881925/

Abstract

PURPOSE

To develop an iron-based solid lipid nanoparticle (SLN) absorbable by the intestinal wall and assess the differential diagnostic value of intestinal lesions in magnetic resonance imaging (MRI).

METHODS

SLNs were prepared with the simultaneous loading of trivalent Fe ions (Fe), levodopa methyl ester (DM), and fluorescein isothiocyanate (FITC). We evaluated the particle size, loading rate, encapsulation efficiency, and cytotoxicity of SLNs. The T contrast effects of the FeDM-FITC-SLNs and gadolinium-based contrast agent (GBCA) were compared in different mouse models: acute ulcerative colitis (AUC), chronic ulcerative colitis (CUC), colon adenocarcinoma (COAD), and normal control. MRI was performed in the same mouse with intravenous injection of GBCA on day 1 and enema of FeDM-FITC-SLNs on day 2. The signal-to-noise ratios (SNRs) were compared using one-way analysis of variance. Tissues were then collected for histology.

RESULTS

The average particle size of FeDM-FITC-SLN was 220 nm. The mean FeDM loading rate was 94.3%, and the encapsulation efficiency was 60.3%. The relaxivity was 4.02 mM·s. After enema with FeDM-FITC-SLNs, MRI showed the following contrast enhancement duration: AUC = COAD > normal > CUC. Confocal fluorescence microscopy confirmed that FeDM-FITC-SLNs were mainly distributed in the intestinal mucosa and tumor capsule.

CONCLUSION

Iron-based SLNs are promising alternatives for contrast enhancement at T1-weighted MRI and will help in the differential diagnosis of intestinal bowel diseases (IBDs).

摘要

目的

开发一种可被肠壁吸收的基于铁的固体脂质纳米粒（SLN），并评估磁共振成像（MRI）中肠病变的鉴别诊断价值。

方法

通过同时载入三价铁离子（Fe）、左旋多巴甲酯（DM）和荧光素异硫氰酸酯（FITC）制备 SLN。我们评估了 SLN 的粒径、载药量、包封率和细胞毒性。在不同的小鼠模型中（急性溃疡性结肠炎（AUC）、慢性溃疡性结肠炎（CUC）、结肠腺癌（COAD）和正常对照组）比较了 FeDM-FITC-SLN 和钆基造影剂（GBCA）的 T 对比效应。在第 1 天静脉注射 GBCA 后和第 2 天进行 FeDM-FITC-SLN 灌肠后，对 MRI 进行了相同的小鼠检查。采用单因素方差分析比较了信噪比（SNR）。然后收集组织进行组织学检查。

结果

FeDM-FITC-SLN 的平均粒径为 220nm。FeDM 的平均载药量为 94.3%，包封率为 60.3%。弛豫率为 4.02mM·s。用 FeDM-FITC-SLN 灌肠后，MRI 显示的对比增强持续时间如下：AUC=COAD>正常>CUC。共聚焦荧光显微镜证实 FeDM-FITC-SLN 主要分布在肠黏膜和肿瘤包膜中。