Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Shandong, 266042, Qingdao, People's Republic of China.
Clinical Laboratory, Qingdao Central Hospital, The Second Affiliated Hospital of Medical College of Qingdao University, Qingdao, 266042, China.
Anal Bioanal Chem. 2022 May;414(11):3529-3539. doi: 10.1007/s00216-022-03979-8. Epub 2022 Mar 1.
Analysis of microRNAs (miRNAs) is important in cancer diagnostics and therapy. Conventional methods used to extract miRNA for analysis are generally time-consuming. A novel approach for rapid and sensitive extraction of miRNAs is urgently need for clinical applications. Herein, a novel strategy based on electrical potential-assisted DNA-RNA hybridization was designed for miRNA extraction. The entire extraction process was accomplished in approximately 3 min, which is much shorter than the commercial adsorption column method, at more than 60 min, or the TRIzol method, at more than 90 min. Additionally, the method offered the advantages of simplicity and specificity during the extraction process by electrical potential-assisted hybridization of single-stranded DNA and RNA. Taking let-7a as an example, satisfactory results were achieved for miRNA extraction in serum, demonstrating the applicability in miRNA nucleic acid amplification.
分析 microRNAs (miRNAs) 在癌症诊断和治疗中非常重要。用于分析 miRNA 的常规方法通常耗时较长。迫切需要一种新的方法来快速、灵敏地提取 miRNA，以满足临床应用的需求。在此，提出了一种基于电势能辅助 DNA-RNA 杂交的新型 miRNA 提取策略。整个提取过程大约需要 3 分钟，比商用吸附柱法（60 分钟以上）或 TRIzol 法（90 分钟以上）快得多。此外，该方法通过电势能辅助单链 DNA 和 RNA 的杂交，在提取过程中具有简单、特异性的优点。以 let-7a 为例，在血清中提取 miRNA 取得了满意的效果，表明该方法适用于 miRNA 核酸扩增。
