由Bst DNA聚合酶催化及窄温循环实现的快速DNA检测和一步式RNA检测
Rapid DNA detection and one-step RNA detection catalyzed by Bst DNA polymerase and narrow-thermal-cycling.
作者信息
Li Mengzhe, Liu Mengmeng, Ma Cuiping, Shi Chao
机构信息
Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, and Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, PR China.
出版信息
Analyst. 2020 Aug 7;145(15):5118-5122. doi: 10.1039/d0an00975j. Epub 2020 Jul 10.
We reported a novel detection method named accelerated strand exchange amplification by employing Bst DNA polymerase and narrow-thermal-cycling for the first time, achieving direct detection of 120 copies of DNA within 15 min and 1.2 × 10 copies of RNA within 20 min and sparking the revolution of the use of routine isothermal polymerases for diverse applications.
我们首次报道了一种名为加速链置换扩增的新型检测方法，该方法采用Bst DNA聚合酶和窄温度循环，能在15分钟内直接检测到120个DNA拷贝，20分钟内检测到1.2×10个RNA拷贝，引发了将常规等温聚合酶用于多种应用的变革。
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