Ocean College, Zhejiang University, Zhoushan, 321000, China.
Beijing Key Laboratory of Drug Target and Screening Research, Beijing, 100050, China.
Chem Biodivers. 2022 Apr;19(4):e202200055. doi: 10.1002/cbdv.202200055. Epub 2022 Feb 28.
In this study, chemical profiles for two co-existing deep-sea-derived Penicillium fungal strains were thoroughly investigated. Two new compounds and 11 known compounds were identified from Penicillium sp. LXY140-R, while one new compound and 12 known compounds were isolated from Penicillium sp. LXY140-3. Their structures were elucidated by extensive 1D and 2D NMR experiments, which were supported by HR-ESI-MS data. The antiproliferative activities of all isolates against HCT-116, A549 and Bel-7402 cell lines were also evaluated. Compounds 2, 5, 6, 10 and 13 showed potent antiproliferative activity. To reveal the metabolic relationship of the two strains, we conducted co-culture experiments to discover cross-talk molecules by a device that allows only small molecule to communicate. Extensive HPLC/MS experiments were applied to identify the disturbance of the chemical profiles within the synthetic Penicillium-Penicillium community. The fungal strain LXY140-R was found to accumulate mono or multiple-acetylation derivatives of deoxynivalenol (DON) sesquiterpenes as responsible molecules by the disturbance of the metabolites produced by the LXY140-3 strain.
在这项研究中，深入研究了两种共存的深海来源的青霉真菌菌株的化学特征。从Penicillium sp. LXY140-R 中鉴定出两种新化合物和 11 种已知化合物，而从 Penicillium sp. LXY140-3 中分离出一种新化合物和 12 种已知化合物。通过广泛的 1D 和 2D NMR 实验确定了它们的结构，这些实验得到了 HR-ESI-MS 数据的支持。还评估了所有分离物对 HCT-116、A549 和 Bel-7402 细胞系的抗增殖活性。化合物 2、5、6、10 和 13 表现出很强的抗增殖活性。为了揭示两种菌株的代谢关系，我们进行了共培养实验，通过一种只允许小分子进行交流的装置发现了交叉对话分子。应用广泛的 HPLC/MS 实验来识别合成青霉-青霉群落中化学特征的干扰。发现真菌菌株 LXY140-R 通过 LXY140-3 菌株产生的代谢物的干扰积累了单或多乙酰化的脱氧雪腐镰刀菌烯醇（DON）倍半萜类化合物作为责任分子。
