Department of Animal Health. NEIKER-Basque Institute for Agricultural Research and Development. Basque Research and Technology Alliance (BRTA), Parque Científico y Tecnológico de Bizkaia, 48160 Derio, Bizkaia, Spain.
Department of Animal Health. NEIKER-Basque Institute for Agricultural Research and Development. Basque Research and Technology Alliance (BRTA), Parque Científico y Tecnológico de Bizkaia, 48160 Derio, Bizkaia, Spain,
J Vector Ecol. 2021 Dec;46(2):173-185. doi: 10.52707/1081-1710-46.2.173.
Studies of the biodiversity of mosquito larval habitats are important for vector-borne disease control programs and help to improve vector distribution maps. This study was designed to investigate the geographical distribution of mosquito species and their larval habitats in urban, rural, and natural areas in northern Spain. Pre-imaginal stages were collected over two sampling periods (spring and summer) in 2019. In the laboratory, immature specimens were reared to the adult stage for species identification by morphological taxonomy and/or molecular methods. In total, 2,182 specimens belonging to 13 different native mosquito species of five genera were collected from 135 sampling points of which 59.2% harbored larvae. s.l. was the most abundant species (45.1%), followed by (12.3%), s.l. (10.2%), (9.5%), and nine other species with lower frequencies that accounted for less than 25%. By molecular identification, s.s. was identified as the only species within the species complex and as the predominant subspecies of the species complex. Margins in large sunlit water bodies were the most suitable sites for s.l., whereas s.l. was present in both natural and artificial habitats. The larval site index, species richness, and relative abundance of the mosquitoes were determined based on the characteristics of the sites where they were collected. The public health importance and ecology of some identified mosquitoes is also discussed.
对蚊虫幼虫栖息地的生物多样性进行研究对于控制媒介传播疾病的项目非常重要，有助于改善媒介分布图。本研究旨在调查西班牙北部城市、农村和自然地区的蚊虫种类及其幼虫栖息地的地理分布。在 2019 年的两个采样期（春季和夏季）中收集了预蛹阶段。在实验室中，通过形态分类学和/或分子方法对未成熟的标本进行饲养，以鉴定成虫种类。总共从 135 个采样点采集了 2182 个标本，属于 5 个属的 13 种本地蚊虫，其中 59.2%有幼虫。 s.l.是最丰富的物种（45.1%），其次是 （12.3%）、 s.l.（10.2%）、 （9.5%）和其他 9 种频率较低的物种，占比不到 25%。通过分子鉴定， s.s.被确定为 物种复合体中的唯一物种， 被确定为 物种复合体中的主要亚种。大型阳光水体边缘是 s.l.最适合的栖息地，而 s.l.则存在于自然和人工栖息地中。根据采集地点的特征，确定了蚊虫的幼虫栖息地指数、物种丰富度和相对丰度。还讨论了一些已确定的蚊子的公共卫生重要性和生态学。
