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比较转录组学分析揭示了不同品种的抗风基因。

Comparative transcriptomics analysis of contrasting varieties of reveals wind resistance genes.

机构信息

China Eucalypt Research Centre, Chinese Academy of Forestry, Zhanjiang, Guangdong, China.

出版信息

PeerJ. 2022 Feb 24;10:e12954. doi: 10.7717/peerj.12954. eCollection 2022.

DOI:10.7717/peerj.12954
PMID:35233295
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8882336/
Abstract

BACKGROUND

Wind, an important abiotic stress factor, affects forests in coastal areas, causes tree damage and timber loss.

METHODS

Two genotypes of strong wind-resistant CA5 and weak wind-resistant C037 were used for RNA-seq analysis to screen for candidate wind-resistance genes and transcription factors (TFs) by comparing the transcriptome analysis of the two varieties in response to wind stress.

RESULTS

It showed that 7061 differentially expressed unigenes could be annotated including 4,110 up-regulated unigenes and 2,951 down-regulated unigenes. Gene Ontology (GO) analysis revealed that six cellulose pathways were involved in response to wind stress. The unigenes in phenylpropanoid biosynthesis, phenylalanine metabolism, and flavonoid biosynthesis pathways were found to be differentially expressed based on Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Moreover, 37 differentially expressed genes were functionally annotated to be involved in the secondary metabolism of phenylalanine (ko00940). Seventy-eight TFs related to the regulating cellulose and lignin synthesis were expressed differently from the various treatments. The expressions of C3H, POX, MYB, NAC, Gene008307, and Gene011799 were significantly upregulated in CA5. Overall, the main response of to wind stress was associated with cell wall biosynthesis; key genes of cellulose and lignin biosynthesis pathways and related TFs were involved in the tree response to wind stress.

摘要

背景

风作为一种重要的非生物胁迫因子，影响沿海地区的森林，导致树木受损和木材损失。

方法

选用抗风能力较强的 CA5 和抗风能力较弱的 C037 两个基因型，通过对两种品种在应对风胁迫时的转录组分析进行比较，筛选候选抗风基因和转录因子（TFs）。

结果

结果显示，有 7061 个差异表达的 unigenes 可以被注释，包括 4110 个上调的 unigenes和 2951 个下调的 unigenes。GO 分析表明，有 6 条纤维素途径参与了对风胁迫的反应。KEGG 分析发现，苯丙烷生物合成、苯丙氨酸代谢和黄酮类生物合成途径中的 unigenes表达存在差异。此外，有 37 个差异表达基因被功能注释为参与苯丙氨酸的次生代谢（ko00940）。78 个与纤维素和木质素合成调节相关的 TF 表达不同，从不同处理中表达。C3H、POX、MYB、NAC、Gene008307 和 Gene011799 在 CA5 中的表达明显上调。总的来说，对风胁迫的主要反应与细胞壁生物合成有关；纤维素和木质素生物合成途径的关键基因及其相关 TF 参与了树木对风胁迫的反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/5afb381e9a1e/peerj-10-12954-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/21172093a452/peerj-10-12954-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/5afb381e9a1e/peerj-10-12954-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/88b99e57578b/peerj-10-12954-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/9a739e12c5dc/peerj-10-12954-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/d344820fd5e6/peerj-10-12954-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/082c34f26c1a/peerj-10-12954-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/ceb6a801a95e/peerj-10-12954-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/6774e93fe097/peerj-10-12954-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/8f3c82a5a915/peerj-10-12954-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/3a778d91cb95/peerj-10-12954-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/06baaef9baa9/peerj-10-12954-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/21172093a452/peerj-10-12954-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6be/8882336/5afb381e9a1e/peerj-10-12954-g011.jpg

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