Pak J Biol Sci. 2022 Jan;25(3):234-244. doi: 10.3923/pjbs.2022.234.244.
<b>Background and Objective:</b> Aflatoxins affect many species including humans and animals, therefore the present study was designed to investigate the protective effect of <i>Chelidonium majus</i> Ethanolic Extract (CMEE) on neurotoxicity induced by Aflatoxin B<sub>1</sub> (AFB1) in rats. <b>Materials and Methods:</b> Four groups of male Albino rats were treated orally for 28 days as follows: (1) Control group was daily given DMSO-PBS buffer (1.0 mL per rat), (2) CMEE (300 mg kg<sup>1</sup>/day) dissolved in DMSO-PBS buffer, (3) AFB1 (80 μg kg<sup>1</sup>/day) dissolved in DMSO-PBS buffer and (4) Received daily AFB1 (300 mg kg<sup>1</sup>) in combination with CMEE (300 mg kg<sup>1</sup>). <b>Results:</b> CMEE exhibits antioxidant activity <i>in vitro</i> and neuroameliorative efficiency <i>in vivo</i> as its administration in combination with AFB1 succeeded significantly in down regulating the elevated levels of inflammatory and apoptotic markers and restoring the values of neurochemical markers (AChE-ase, dopamine and serotonin) that were deteriorated by AFB1 intake. <b>Conclusion:</b> In conclusion, the neuroprotective effect of CMEE may be mediated through its antioxidant and free radical scavenging activity that proved from the data<i> </i>of ferric-reducing power ability and DPPH radical scavenging activity.
<b>背景与目的：</b>黄曲霉毒素影响包括人类和动物在内的许多物种，因此本研究旨在探讨 <i>Chelidonium majus</i> 乙醇提取物（CMEE）对黄曲霉毒素 B<sub>1</sub>（AFB1）诱导的大鼠神经毒性的保护作用。<b>材料与方法：</b>四组雄性白化大鼠经口给予以下药物治疗 28 天：（1）对照组每日给予 DMSO-PBS 缓冲液（1.0 mL/只），（2）CMEE（300 mg/kg<sup>1</sup>/天）溶于 DMSO-PBS 缓冲液中，（3）AFB1（80 μg/kg<sup>1</sup>/天）溶于 DMSO-PBS 缓冲液中，（4）每日接受 AFB1（300 mg/kg<sup>1</sup>）与 CMEE（300 mg/kg<sup>1</sup>）联合治疗。<b>结果：</b>CMEE 在体外表现出抗氧化活性，在体内具有神经改善作用，因为其与 AFB1 联合使用可显著下调升高的炎症和凋亡标志物水平，并恢复因摄入 AFB1 而恶化的神经化学标志物（乙酰胆碱酯酶、多巴胺和 5-羟色胺）的水平。<b>结论：</b>总之，CMEE 的神经保护作用可能是通过其抗氧化和自由基清除活性介导的，这从铁还原能力和 DPPH 自由基清除活性的数据中得到了证明。
