Segneanu Adina-Elena, Vlase Gabriela, Vlase Titus, Ciocalteu Maria-Viorica, Bejenaru Cornelia, Buema Gabriela, Bejenaru Ludovic Everard, Boia Eugen Radu, Dumitru Andrei, Boia Simina
Institute for Advanced Environmental Research-West, University of Timisoara (ICAM-WUT), Oituz nr. 4, 300223 Timisoara, Romania.
Research Center for Thermal Analysis for Environmental Problems, West University of Timisoara, Pestalozzi St. 16, 300115 Timisoara, Romania.
Plants (Basel). 2024 Mar 5;13(5):734. doi: 10.3390/plants13050734.
Novel nanotechnology based on herbal products aspires to be a high-performing therapeutic platform. This study reports the development of an original engineering carrier system that jointly combines the pharmacological action of and AuNPs, with unique properties that ensure that the limitations imposed by low stability, toxicity, absorption, and targeted and prolonged release can be overcome. The metabolite profile of Romanian wild-grown contains a total of seventy-four phytochemicals belonging to eight secondary metabolite categories, including alkaloids, amino acids, phenolic acids, flavonoids, carotenoids, fatty acids, sterols, and miscellaneous others. In this study, various techniques (XRD, FTIR, SEM, DLS, and TG/DTG) were employed to investigate his new carrier system's morpho-structural and thermal properties. In vitro assays were conducted to evaluate the antioxidant potential and release profile. The results indicate 99.9% and 94.4% dissolution at different pH values for the CG-AuNPs carrier system and 93.5% and 85.26% for greater celandine at pH 4 and pH 7, respectively. Additionally, three in vitro antioxidant assays indicated an increase in antioxidant potential (flavonoid content 3.8%; FRAP assay 24.6%; and DPPH 24.4%) of the CG-AuNPs carrier system compared to the herb sample. The collective results reflect the system's promising perspective as a new efficient antimicrobial and anti-inflammatory candidate with versatile applications, ranging from target delivery systems, oral inflammation (periodontitis), and anti-age cosmetics to extending the shelf lives of products in the food industry.
基于草药产品的新型纳米技术有望成为一个高性能的治疗平台。本研究报告了一种原创工程载体系统的开发，该系统将[草药名称]的药理作用与金纳米粒子（AuNPs）相结合，具有独特的性能，可确保克服低稳定性、毒性、吸收以及靶向和缓释所带来的限制。罗马尼亚野生[草药名称]的代谢产物谱总共包含74种植物化学物质，属于八个次生代谢物类别，包括生物碱、氨基酸、酚酸、黄酮类、类胡萝卜素、脂肪酸、甾醇和其他各类物质。在本研究中，采用了各种技术（X射线衍射、傅里叶变换红外光谱、扫描电子显微镜、动态光散射和热重/微商热重）来研究这种新型载体系统的形态结构和热性能。进行了体外试验以评估抗氧化潜力和释放曲线。结果表明，CG-AuNPs载体系统在不同pH值下的溶出率分别为99.9%和94.4%，而白屈菜在pH 4和pH 7时的溶出率分别为93.5%和85.26%。此外，三项体外抗氧化试验表明，与草药样品相比，CG-AuNPs载体系统的抗氧化潜力有所提高（黄酮类含量提高3.8%；铁离子还原抗氧化能力测定提高24.6%；二苯基苦味酰基自由基测定提高24.4%）。这些综合结果反映了该系统作为一种新型高效抗菌和抗炎候选物的广阔前景，具有广泛的应用，从靶向递送系统、口腔炎症（牙周炎）、抗老化化妆品到延长食品工业产品的保质期。
