Feng Dao, Gao Jiaoqi, Gong Zhiwei, Zhou Yongjin J
School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430080, Hubei, China.
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Feb 25;38(2):760-771. doi: 10.13345/j.cjb.210102.
Fatty acids (FA) are widely used as feed stocks for the production of cosmetics, personal hygiene products, lubricants and biofuels. is considered as an ideal chassis for bio-manufacturing, due to its outstanding characteristics such as methylotroph, thermal-tolerance and wide substrate spectrum. In this study, we harnessed for overproduction of fatty acids by engineering its fatty acid metabolism and optimizing the fermentation process. The engineered strain produced 1.86 g/L FAs under the optimized shake-flask conditions (37℃, pH 6.4, a C/N ratio of 120 and an of seed culture of 6-8). The fed-batch fermentation process was further optimized by using a dissolved oxygen (DO) control strategy. The C/N ratio of initial medium was 17.5, and the glucose medium with a C/N ratio of 120 was fed when the DO was higher than 30%. This operation resulted in a titer of 18.0 g/L FA, indicating the potential of using as an efficient cell factory for the production of FA.
脂肪酸（FA）被广泛用作生产化妆品、个人卫生用品、润滑剂和生物燃料的原料。由于其具有甲基营养型、耐热性和广泛的底物谱等突出特性，被认为是生物制造的理想底盘。在本研究中，我们通过改造其脂肪酸代谢并优化发酵过程，利用其过量生产脂肪酸。在优化的摇瓶条件（37℃、pH 6.4、碳氮比为120以及种子培养物的光密度为6 - 8）下，工程菌株产生了1.86 g/L的脂肪酸。通过使用溶解氧（DO）控制策略进一步优化了分批补料发酵过程。初始培养基的碳氮比为17.5，当溶解氧高于30%时，补加碳氮比为120的葡萄糖培养基。该操作使脂肪酸产量达到18.0 g/L，表明利用其作为生产脂肪酸的高效细胞工厂的潜力。
