Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USA.
Phys Chem Chem Phys. 2022 Mar 16;24(11):6410-6414. doi: 10.1039/d2cp00689h.
Immobilized avidin-biotin complexes were used to release biotinylated (bio)molecules upon producing local pH changes near an electrode surface by electrochemical reactions. The nitro-avidin complex with biotin was dissociated by increasing local pH with electrochemical O reduction. The avidin complex with iminobiotin was split by decreasing local pH with electrochemical oxidation of ascorbate. Both studied systems were releasing molecule cargo species in response to small electrical potentials (-0.4 V or 0.2 V for the O reduction or ascorbate oxidation, respectively) applied on the modified electrodes.
固定化亲和素-生物素复合物可通过电化学反应在电极表面附近产生局部 pH 变化，从而释放生物素化（bio）分子。通过电化学 O 还原增加局部 pH 可使带负电荷的亲和素-生物素复合物解离。通过电化学氧化抗坏血酸降低局部 pH 可使带正电荷的亲和素-亚氨基生物素复合物分裂。两种研究体系都能响应施加在修饰电极上的小电势（O 还原或抗坏血酸氧化的电位分别为-0.4 V 或 0.2 V）释放分子载体物种。
