Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, United States.
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, United States.
Front Cell Infect Microbiol. 2022 Feb 14;12:833269. doi: 10.3389/fcimb.2022.833269. eCollection 2022.
There is a critical need to accurately diagnose, prevent, and treat biofilms in humans. The biofilm forming bacteria can cause acute and chronic infections, which are difficult to treat due to their ability to evade host defenses along with an inherent antibiotic-tolerance. Using an untargeted NMR-based metabolomics approach, we identified statistically significant differences in 52 metabolites between grown in the planktonic and lawn biofilm states. Among them, the metabolites of the cadaverine branch of the lysine degradation pathway were systematically decreased in biofilm. Exogenous supplementation of cadaverine caused significantly increased planktonic growth, decreased biofilm accumulation by 49% and led to altered biofilm morphology, converting to a pellicle biofilm at the air-liquid interface. Our findings show how metabolic pathway differences directly affect the growth mode in and could support interventional strategies to control biofilm formation.
准确诊断、预防和治疗人体生物膜存在着迫切需求。形成生物膜的细菌会引起急性和慢性感染，由于其逃避宿主防御的能力以及固有的抗生素耐药性，这些感染很难治疗。本研究采用无靶向核磁共振代谢组学方法，在浮游和菌毯生物膜状态下生长的细菌之间确定了 52 种代谢物存在统计学显著差异。其中，赖氨酸降解途径中的腐胺分支的代谢物在生物膜中系统减少。腐胺的外源性补充导致浮游生长显著增加，生物膜积累减少 49%，并导致生物膜形态发生改变，在气液界面形成菌膜。我们的研究结果表明代谢途径差异如何直接影响 的生长模式，并为控制生物膜形成的干预策略提供支持。
