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通过抑制乙酰胆碱酯酶和氧化应激改善东莨菪碱诱导的小鼠记忆损伤。

Ameliorates Scopolamine-Induced Memory Impairment in Mice through Inhibition of Acetylcholinesterase and Oxidative Stress.

作者信息

Al-Amin Md Yusuf, Lahiry Amitav, Ferdous Rafia, Hasan Md Kamrul, Kader Md Abdul, Alam Ahm Khurshid, Saud Zahangir Alam, Sadik Md Golam

机构信息

Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.

Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh.

出版信息

Adv Pharmacol Pharm Sci. 2022 Feb 21;2022:8305271. doi: 10.1155/2022/8305271. eCollection 2022.

DOI:10.1155/2022/8305271
PMID:35237756
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8885280/
Abstract

Alzheimer's disease (AD) is a progressive neurological disorder characterized by loss of memory and cognition. is being used as traditional medicine in the treatment of different neurological problems. In this study, we evaluated the anticholinesterase and antioxidant activities of the crude methanol extract of and its fractions and the neuroprotective effect of the most active fraction in the scopolamine-induced mouse model of memory impairment. Among the crude extract and its fractions, chloroform fraction exerted strong inhibition of acetylcholinesterase and butyrylcholinesterase enzymes with IC values of 40.06 and 18.78 g/mL, respectively. Similarly, the chloroform fraction exhibited potent antioxidant activity and effectively inhibited the peroxidation of brain lipid in vitro. The phytochemical profile revealed the high content of polyphenolics and alkaloids in the chloroform fraction. Pearson's correlation studies showed a significant association of anticholinesterase and antioxidant activity with alkaloid and phenolic contents. Kinetic analysis showed that the chloroform fraction exhibited a noncompetitive type of inhibition. In experimental mice, the chloroform fraction restored the impaired learning and memory induced by scopolamine as evidenced by a significant decrease in latency time and increase of quadrant time in probe trial in Morris water maze task. The chloroform fraction also significantly reduced the activity of acetylcholinesterase and oxidative stress in mice. Our results suggest that the chloroform fraction of may represent a potential candidate for the prevention and treatment of AD.

摘要

阿尔茨海默病（AD）是一种以记忆和认知丧失为特征的进行性神经疾病。 正被用作治疗不同神经问题的传统药物。在本研究中，我们评估了 的粗甲醇提取物及其馏分 的抗胆碱酯酶和抗氧化活性，以及在东莨菪碱诱导的记忆损伤小鼠模型中最具活性馏分的神经保护作用。在粗提取物及其馏分中，氯仿馏分对乙酰胆碱酯酶和丁酰胆碱酯酶具有强烈抑制作用，IC值分别为40.06和18.78μg/mL。同样，氯仿馏分表现出强大的抗氧化活性，并在体外有效抑制脑脂质过氧化。植物化学分析表明氯仿馏分中多酚和生物碱含量高。Pearson相关性研究表明抗胆碱酯酶和抗氧化活性与生物碱和酚类含量之间存在显著关联。动力学分析表明氯仿馏分表现出非竞争性抑制类型。在实验小鼠中，氯仿馏分恢复了东莨菪碱诱导的受损学习和记忆，这在莫里斯水迷宫任务的探针试验中潜伏期时间显著缩短和象限时间增加得到证明。氯仿馏分还显著降低了小鼠体内乙酰胆碱酯酶的活性和氧化应激。我们的结果表明， 的氯仿馏分可能是预防和治疗AD的潜在候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/952b10edd469/APS2022-8305271.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/84f0db2fee5f/APS2022-8305271.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/48a785245204/APS2022-8305271.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/952b10edd469/APS2022-8305271.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/84f0db2fee5f/APS2022-8305271.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/ec8174dbaa1c/APS2022-8305271.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/64355fb9c2b1/APS2022-8305271.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/ccb186995413/APS2022-8305271.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/27ccf8315280/APS2022-8305271.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/48a785245204/APS2022-8305271.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047e/8885280/952b10edd469/APS2022-8305271.007.jpg

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