Department of Molecular Pathology, Faculty of Medicine, Interdisciplinary Graduate School of Medicine, University of Yamanashi, Chuo, Japan.
School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China.
Methods Mol Biol. 2022;2419:413-431. doi: 10.1007/978-1-0716-1924-7_25.
Rabbits are a useful animal model for examining human hyperlipidemia and atherosclerosis because they have unique features of lipoprotein metabolism that are similar to those in humans. Feeding rabbits a cholesterol-rich diet is a simple means to induce experimental atherosclerosis. Indeed, cholesterol-fed rabbits were first applied to address the relationship between dietary cholesterol and atherosclerosis more than 100 years ago. However, the methods for investigating atherosclerosis using cholesterol-fed rabbits have not been well formulated. In this chapter, we attempt to provide readers with the essential methods to use cholesterol-fed rabbits in the examination of atherosclerosis from cholesterol diet preparation to lesion analysis. These protocols are compiled for both experienced and young researchers who intend to use rabbits for the investigation of lipoprotein metabolism and atherosclerosis.
兔子是研究人类高脂血症和动脉粥样硬化的有用动物模型，因为它们具有与人类相似的脂蛋白代谢的独特特征。用富含胆固醇的饮食喂养兔子是诱导实验性动脉粥样硬化的简单方法。事实上，用胆固醇喂养兔子的方法早在 100 多年前就首次被应用于研究饮食胆固醇与动脉粥样硬化之间的关系。然而，用胆固醇喂养兔子研究动脉粥样硬化的方法尚未得到很好的制定。在这一章中，我们试图为读者提供必要的方法，从胆固醇饮食准备到病变分析，使用胆固醇喂养的兔子来检查动脉粥样硬化。这些方案是为有经验的和年轻的研究人员编写的，他们打算使用兔子来研究脂蛋白代谢和动脉粥样硬化。
